I cannot see CRISPR-Cas9 editing. What can I do?

Jul 31, 2017, 06:00 AM
  • Double-check that the your crRNA sequence matches the species and target location as expected. For example, verify that the target site in your cells does not show any polymorphism that could affect the potency of the crRNA.
  • Confirm that you can observe successful editing using a known positive control guide RNA, such as the Alt-R® positive control crRNAs for human, mouse, or rat.
  • If the positive controls work but the experimental crRNA does not, test additional target sites nearby to see if a more efficient guide RNA can be found.

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