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What DsiRNA concentration do I need to use in my experiment to see knockdown?

Jul 29, 2013, 18:59 PM
For an RNAi or antisense experiment, the actual level of target gene knockdown is related to the transfection efficiency. A positive control such as HPRT DsiRNA should be used in each experiment to assess transfection efficiency.  In addition, IDT recommends using a dose-response curve of 0.1, 1, and 10 nM to determine maximum response.
Categories:
  • Gene Silencing and Small RNAs
  • Gene Silencing and Small RNAs
Tags:
  • DsiRNA
  • transfection