Do the positive and negative controls in the TriFECTa Kit come with primers and RT-PCR materials?
The TriFECTa® Kit includes: a) 3 Dicer-Substrate RNAi duplexes (2 nmoles each). b) 1 negative control duplex: NC1, a scrambled sequence. c) 1 positive control duplex: HPRT-S1 DS Positive Control. d) 1 fluorescent transfection control duplex: TYE 563-labeled transfection control (formerly Cy3 DS Transfection Control). e) HPLC Purification Duplex Resuspension Buffer. The kit does not include primers or PCR reagents for quantification of gene silencing.