Top Questions

How can I ensure I am designing my CRISPR-Cpf1 crRNA guide sequence in the correct orientation and to the appropriate strand? What sequence should be input into the CRISPR-Cpf1 crRNA ordering tool?

Enter the 20–24 base DNA sequence downstream (3’ end) of the target Cpf1 PAM site (TTTV*), and then you are ready to continue. We typically recommend using a 21-nucleotide guide sequence.

The ordering tool will automatically convert the DNA sequence to RNA during the ordering process. If you are pasting your Cpf1 target site from an online design tool, make sure to verify the correct strand orientation before pasting the target sequence.

* V = A, C, or G

Tags:
  • Cpf1
  • CRISPR
  • Alt-R
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