Top Questions

How can I estimate the amount of editing obtained using the Alt-R® CRISPR-Cas9 or -Cpf1 Systems?

We recommend using the Alt-R® Genome Editing Detection Kit to determine the relative amount of editing obtained. This method uses T7 endonuclease I (T7EI) to cleave heterodimers formed in PCR amplicons following CRISPR editing.

It is important to consider that the T7EI-based assay underestimates the amount of total editing, as it does not accurately account for single-base insertions or deletions, but rather detects indels of multiple bases. Nonetheless, the Alt-R Genome Editing Detection Kit is the recommended approach for estimating editing efficiency, because of its ease of use and cost-efficiency compared to Surveyor® endonuclease assays or Sanger sequencing.


Tags:
  • Cpf1
  • Cas9
  • mutation detection kits
  • CRISPR
  • Alt-R
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