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Top Questions

Which dye label provides the brightest signal and where in the oligo should it be positioned?

The dyes with the most intense signals that IDT offers are the Alexa Fluor and ATTO dye classes of fluorescent dyes. Signal intensity is not effected by  position within the oligo sequence. Green, yellow, and some orange dyes can be partially quenched by guanine bases. Therefore, if you decide to order a dye in that range, you should try not to position the dye immediately next to a guanine base.  Read more details about this quenching effect. 
Tags:
  • fluor
  • dye
  • Alexa
  • ATTO
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