Top Questions

Do I need to worry about performance if my shipment of PrimeTime® Gene Expression Master Mix is delayed?

No. IDT has tested 3 batches of PrimeTime master mix by heating at 50°C for 1, 3, or 7 days before use in qPCR experiments (Figure). High PCR efficiency was observed (range of 95.1–103.6% with R2 >0.991) with the assays tested (HPRT and GUSB). When comparing the controls (containing frozen, unheated master mix) to reactions containing heat-treated master mix, ΔCq values were <0.5, with a few exceptions that were primarily seen when using a low amount of starting template. The small ΔCq values emphasize the stability and robustness of PrimeTime master mix after extreme thermal stress treatment.

qPCR stability at 50 degrees, up to 7 days

Figure. Consistent, high PCR efficiency and no change in Cq values, even after PrimeTime® Gene Expression Master Mix is heated to 50°C for up to 7 days. PrimeTime master mix was incubated at 50°C for 1, 3, 5, or 7 days, while a control master mix sample was stored frozen (–20°C) until use. The graph shows representative amplification curves from qPCRs that included the heat-treated or control master mix with PrimeTime qPCR HPRT Assays (Assay ID: Hs.PT.58v.45621572). The reactions remained at room temperature for 24 hr before running the thermal cycler: control master mix (gray) and the 1-, 3-, 5-, and 7-day, heat-treated master mix (green, light blue, dark blue, and orange, respectively).

For more performance testing data on ambient temperature shipping, see the white paper, Exceptional thermal stability of PrimeTime® Gene Expression Master Mix enables ambient shipping, saving laboratory and environmental resources.

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  • qPCR master mix
  • qPCR
  • primetime
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