Top Questions

What is the optimal length of a primer?

IDT recommends you aim for primers between 18–30 bases; however the most important considerations for primer design should be their Tm value and specificity. Primers should also be free of strong secondary structures and self-complementarity.  The free IDT OligoAnalyzer SciTool can be used to evaluate these features.
  • Tm
  • Oligoanalyzer
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