The Tm value reported on our spec sheets uses a 25 µM oligonucleotide and a 50 mM salt concentration. It does not take into account dNTP or Mg2+ concentrations. To get an accurate Tm for your specific application, all of these concentrations should be input and adjusted to match the reaction conditions you plan to use. The free IDT OligoAnalyzer SciTool (www.idtdna.com/scitools
) calculates Tm for the concentrations you imput.