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Top Questions

Should I avoid purification for sequences containing mixed bases (degenerate or randomized oligos)?

The decision to order purified oligos will vary by your research needs. Purification involves collecting fractions of the total product synthesized and will inherently reduce variability in the oligos, possibly skewing the random base ratios, resulting in less overall randomness. Taking this into consideration, there are some cases where purification may still be warranted, especially with very long or highly modified oligos. If you have additional questions regarding purification of your mixed base oligos, contact customer service at custcare@idtdna.com.
Tags:
  • random
  • degenerate
  • Mixed
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