Top Questions

Should I only be concerned about sodium ions when calculating Tm?

Any charge will play a role in the Tm value for an oligonucleotide in a reaction. Thus, sodium, magnesium, and potassium concentrations each contribute to this calculation. The IDT OligoAnalyzer® tool will calulate Tm for you, taking into account the presence of specific ions in the reaction buffer. Find this program under the "SciTools®" tab of the IDT website.
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