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Can you use gBlocks® Gene Fragments to alter restriction sites in a plasmid vector?

Because each gBlocks Gene Fragment can be 125−2000 bp in length, the fragments can be designed to replace a segment of vector up to 2 kb in size (allowing for 20–30 bp overlapping regions between the gene fragment and plasmid insertion site) using the Gibson Assembly® Method. This would make possible the replacement of many restriction sites, as well as adding tags and other useful sequences. Learn more about gBlocks Gene Fragments.

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  • restriction
  • gBlocks
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