We are upgrading your website experience! Click here for more information. TechVault PRODUCT DNA/RNA Synthesis Gene Silencing and Small RNAs Genotyping GMP Manufacturing Modifications Molecular Biology Reagents NextGen Sequencing PCR/qPCR Purification QC Information RNase H2 SciTools Applications Synthetic Biology APPLICATION Cloning and Fragment Assembly Gene Silencing and Small RNAs General Oligo Use Genotyping Mutagenesis NextGen Sequencing Oligo Characteristics PCR/qPCR CUSTOMER SUPPORT Account Ordering Pricing and Payment Shipping and Turnaround FAQ/ Tags Can BLAST searhes be performed with the OligoAnalyzer® program? How do I calculate the molecular weight of an oligo? How do I determine the Tm of my sequence? How do I use the OligoAnalyzer tool to analyze possible hairpins and dimers formed by my oligo? How does IDT caclculate extinction coefficient (ɛ) and melting temperature (Tm)? What molar concentrations of oligonucleotide and salts are used to calculate the Tm reported on specification sheets? What secondary structure considerations need to be included when designing primers for PCR? Why is the Tm provided on your oligonucleotide specification sheets different from the calculated Tm I obtained when I designed my oligos?