We are upgrading your website experience! Click here for more information. TechVault PRODUCT DNA/RNA Synthesis Gene Silencing and Small RNAs Genotyping GMP Manufacturing Modifications Molecular Biology Reagents NextGen Sequencing PCR/qPCR Purification QC Information RNase H2 SciTools Applications Synthetic Biology APPLICATION Cloning and Fragment Assembly Gene Silencing and Small RNAs General Oligo Use Genotyping Mutagenesis NextGen Sequencing Oligo Characteristics PCR/qPCR CUSTOMER SUPPORT Account Ordering Pricing and Payment Shipping and Turnaround FAQ/ Tags How can I design dual-labeled probes? How do I dilute and store PrimeTime qPCR Probes? How much cDNA is recommended per PrimeTime qPCR Assay reaction? How should I determine the annealing temperature for a probe-based qPCR assay? Should I select a Tm midway between my primer and probe Tms? What concentrations are the primers and probe in the PrimeTime® qPCR Assay? What master mix does IDT recommend for use with PrimeTime® qPCR Assays? Which dyes are available for use with the IDT ZEN quencher?