TechVault PRODUCT DNA/RNA Synthesis Gene Silencing and Small RNAs Genome Editing Genotyping GMP Manufacturing Modifications Molecular Biology Reagents NextGen Sequencing PCR/qPCR Purification QC Information RNase H2 SciTools Applications Synthetic Biology APPLICATION Cloning and Fragment Assembly Gene Silencing and Small RNAs General Oligo Use Genome Editing Genotyping Mutagenesis NextGen Sequencing Oligo Characteristics PCR/qPCR CUSTOMER SUPPORT Account Ordering Pricing and Payment Shipping and Turnaround FAQ/ Tags How can I design dual-labeled probes? How do I dilute and store PrimeTime qPCR Probes? How much cDNA is recommended per PrimeTime qPCR Assay reaction? How should I determine the annealing temperature for a probe-based qPCR assay? Should I select a Tm midway between my primer and probe Tms? What concentrations are the primers and probe in the PrimeTime® qPCR Assay? Which dyes are available for use with the IDT ZEN quencher?