TECHVAULT > READING ROOM > QUICK REFERENCE

Mixed Bases Definitions

Mixed bases, which are also known as degenerate or wobble bases, can be introduced at any position in an oligomer sequence. For example, mixed base composition at a single position can include all 4 bases ("N"), C or T bases ("Y"), A or G bases ("R"), etc. Since there are 11 different possible combinations of 2, 3 or 4 bases, a universal nomenclature has been established that must be used when specifying nucleic acid content as a mixed base site.

IUB Codes:

Symbol Mixed Bases
R A,G
Y C,T
M A,C
K G,T
S C,G
W A,T
H A,C,T
B C,G,T
V A,C,G
D A,G,T
N A,C,G,T