Troubleshooting qPCR - What Are My Amplification Curves Telling Me?

Quantitative PCR (qPCR) is the method of choice for accurate estimation of gene expression. Part of its appeal for researchers comes from having a protocol that is easy to execute. However when your reactions do not result in ideal amplification, troubleshooting "why" can be challenging. Factors including sample quality, template quantity, master mix differences, assay design, and incorrect primer or probe resuspension can all influence efficient amplification. When troubleshooting, analysis of the appearance of your amplification curve can give you clues towards improving your results. This webinar will present a variety of problematic qPCR issues and how they are manifested in the amplification curve.

About the Speaker

Aurita Menezes, Ph.D. began her science education in the field of biochemistry and went on to obtain a Ph.D. in microbiology. Her postdoctoral work focused on the effects of viral and chemical injury to pulmonary cells. Throughout her research career she has used a broad range of molecular techniques including qPCR 5' nuclease assays. Aurita is currently a Scientific Applications Specialist at IDT and, as an authority on IDT PrimeTime® qPCR products, she trains customer care and technical support representatives on the basics of PCR and qPCR. In addition, she is responsible for designing researchers' qPCR assays, troubleshooting their experiments, and supporting researchers in analysis and interpretation of their qPCR data.