Planning and Executing In Vitro siRNA Experiments

Functional analysis by mRNA knockdown using siRNAs is now routine in many molecular biology labs. However, many RNAi experiments fail due to diversion from simple, good practices. This webinar reviews the steps leading to successful siRNA experiments, including: 

  • Understanding the target transcript
  • siRNA selection
  • Choosing the cell type
  • Validating the assay
  • Including appropriate biological controls

Presentation slides: