How to Design qPCR Primers Spanning Exon Junctions Using PrimerQuest

This tutorial demonstrates how to use PrimerQuest to design PCR or qPCR primers that span exon junctions for a gene. PrimerQuest is one of IDT's scientific design and analysis tools. It is the design tool of choice for custom design of primers and probes PCR, qPCR, and sequencing.