State-of-the-Art Normalization of RT-qPCR Data

Presented by Dr Jo Vandesompele

State-of-the-Art Normalization of RT-qPCR Data

Setting up a qPCR experiment is so simple that it actually becomes dangerous. Without appropriate controls and data normalization, results can be misleading at best. During this webinar, Dr Vandesompele addresses selection and validation of suitable reference genes as well as the use of the global mean normalization method to obtain accurate data. He also describes tools for data generation and analysis. At the end of the webinar, Dr Vandesompele provides access to a free, 90+ page qPCR Application Guide and discount on analysis software. 

About the Speaker: Dr Jo Vandesompele

Dr Jo Vandesompele is a professor of functional genomics and applied bioinformatics at Ghent University. He has written numerous ground-breaking publications on normalization of gene expression and real-time PCR data analysis, and co-authored the MIQE guidelines for publication of qPCR experiments. With Dr Jan Hellemans, Dr Vandesompele cofounded Biogazelle, a real-time PCR company, built upon a decade of experience in real-time PCR experiment design, assay development, and data-analysis (

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IDT and Biogazelle are working together to provide users of PrimeTime® qPCR products a solution for qPCR data analysis. qbasePLUS software from Biogazelle is a qPCR analysis package that allows direct import of data from qPCR instruments from a variety of manufacturers. qbasePLUS software offers statistical tools for qPCR data analysis and graphical presentation tools for viewing analyzed data. It is the most powerful, flexible, and user-friendly real-time PCR data analysis software available.

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