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When You Need to Customize PCR/qPCR Primer Designs
It is often necessary to select primers using specific parameters; e.g., a particular GC content or Tm, forcing the start or stop position of a primer end. However, not all primer design tools provide this flexibility. In this interactive webinar, we will demonstrate several primer customization scenarios using the free, highly popular PrimerQuest™ Design tool including:
- When to use default design parameters, and when to customize assay design
- How to design primers for one, two, or up to 50 sequences at a time
- How to approach specific customized design scenarios suggested by and voted on by participants of this webinar