{"id":813,"date":"2011-09-12T16:48:00","date_gmt":"2011-09-12T16:48:00","guid":{"rendered":"https:\/\/www.idtdna.com\/page\/oligo-modification-post-synthesis-conjugation-explained"},"modified":"2025-08-22T16:06:33","modified_gmt":"2025-08-22T16:06:33","slug":"oligo-modification-post-synthesis-conjugation-explained","status":"publish","type":"post","link":"https:\/\/www.idtdna.com\/page\/support-and-education\/decoded-plus\/oligo-modification-post-synthesis-conjugation-explained\/","title":{"rendered":"Oligo modification\u2014Post-synthesis conjugation explained"},"content":{"rendered":"<h2>Phosphoramidite synthesis vs. post-synthesis conjugation<\/h2>\n<p>Modifications can be incorporated into synthetic oligonucleotides in a variety of ways. Standard DNA bases are synthetically coupled via phosphoramidite chemistry. The reaction proceeds in the 3' to 5' direction where the 5' hydroxyl group of each base attaches to the 3' phosphate group of the next base. Many modifications, such as 6-FAM, standard biotin, and internal Cy3, can be attached via phosphoramidite chemistry directly on the synthesis column (Figure&nbsp;1). <\/p>\n<figure class=\"wp-block-image\"><img decoding=\"async\" src=\"https:\/\/www.idtdna.com\/page\/wp-content\/uploads\/idt-images\/cd255a15-3279-6e2e-aa53-ff00001c1b3c-postsynth-conj_fig-1.jpg\" data-displaymode=\"Original\" alt=\"Internal Cy3 modification\" title=\"D-OLI12PS-Postsynth conj-F1\" \/><figcaption class=\"image-caption\">Figure 1. Internal Cy3 modification. Standard phosphoramidite chemistry attaches the modification through the hydroxyl group (arrow H) and the phosphate group (arrow P) to neighboring bases.<\/figcaption><\/figure>\n<\/p>\n<p>Most modifications are attached using phosphoramidite chemistry. However, some modifications, such as NHS esters and click chemistry modifications, are attached post synthesis. The following sections will focus on the common post-synthesis conjugations performed at IDT.<\/p>\n<h2>NHS ester modifications<\/h2>\n<p>NHS ester modifications contain an NHS (N-hydroxysuccinimide) group that reacts with an amine group to form an amide (Figure&nbsp;2). <\/p>\n<figure class=\"wp-block-image\"><img decoding=\"async\" src=\"https:\/\/www.idtdna.com\/page\/wp-content\/uploads\/idt-images\/cd255a15-3279-6e2e-aa53-ff00001c1b3c-postsynth-conj_fig-2.jpg\" data-displaymode=\"Original\" alt=\"standard NHS ester modification reaction\" title=\"D-OLI12PS-Postsynth conj-F2\" \/><figcaption class=\"image-caption\">Figure 2. Standard NHS ester modification reaction.<\/figcaption><\/figure>\n<\/p>\n<p>Typically, 5' NHS esters are attached through an Amino Modifier C6 group, internal NHS esters through an Amino Modifier C6 dT, and 3' NHS esters via an amino group linked to the controlled pore glass (CPG) beads used as the synthesis support (Figure&nbsp;3).When ordering modified oligos, certain modifications like fluorescent dyes may have to be attached to the oligo via the NHS ester attachment route. For example, 5' MAX&trade; or 5' JOE&trade; will be designated as 5'&nbsp;MAX (NHS Ester) or 5'&nbsp;JOE (NHS Ester) when ordered. <\/p>\n<figure class=\"wp-block-image\"><img decoding=\"async\" src=\"https:\/\/www.idtdna.com\/page\/wp-content\/uploads\/idt-images\/cd255a15-3279-6e2e-aa53-ff00001c1b3c-postsynth-conj_fig-3.jpg\" data-displaymode=\"Original\" alt=\"amino modifiers used to attach NHS esters\" title=\"D-OLI12PS-Postsynth conj-F3\" \/><figcaption class=\"image-caption\">Figure 3. Amino modifiers used to attach NHS esters. The 3' Amino Modifier (not shown) is similar in structure to the 5' Amino Modifier but is attached to the solid support (controlled pore glass, or CPG) on which the oligos are synthesized.<\/figcaption><\/figure>\n<\/p>\n<h2>What is click chemistry?<\/h2>\n<p>The nature and mechanism of click chemistry was described by Dr K.&nbsp;B.&nbsp;Sharpless at the Scripps Institute in 2001 [<a href=\"#references\">1,2<\/a>]. A click chemistry reaction entails coupling azide and alkyne groups through a copper-catalyzed reaction, forming a 1,2,3-triazole. This reaction is thermodynamically favorable, resulting in an irreversible reaction with no side products (Figure&nbsp;4). There are also copper-free click reactions [<a href=\"#references\">3<\/a>]. <\/p>\n<figure class=\"wp-block-image\"><img decoding=\"async\" src=\"https:\/\/www.idtdna.com\/page\/wp-content\/uploads\/idt-images\/cd255a15-3279-6e2e-aa53-ff00001c1b3c-postsynth-conj_fig-4.jpg\" data-displaymode=\"Original\" alt=\"basic click chemistry reaction\" title=\"D-OLI12PS-Postsynth conj-F4\" \/><figcaption class=\"image-caption\">Figure 4. Basic click chemistry reaction. The copper catalyzed reaction between an azide group and an alkyne group produces a 1,2,3-triazole. IDT attaches a variety of modifications post synthesis using this click chemistry reaction.<\/figcaption><\/figure>\n<\/p>\n<p>IDT offers a variety of oligo modifications that leave a free azide or alkyne group available for further click conjugation, giving researchers the freedom to conjugate molecules of their choice to create custom oligo modifications. Alternatively, IDT can do the click conjugation for you by including a reactive alkyne group in the oligonucleotide during synthesis. After synthesis, deprotection, and initial purification, the alkyne group is reacted with a modification containing an azide functional group. All 5' click modifications conjugate through a 5' hexynyl group (Figure&nbsp;5A) while internal click modifications conjugate through an internal alkyne (Figure&nbsp;5B). The internal alkyne group is attached to dT, meaning any internal modification attached via click chemistry will incorporate an additional T base into the oligonucleotide sequence. To identify oligo modifications attached via click chemistry, look for (azide) after the modification name, such as 6-FAM (azide). IDT can also provide a 3' Alkyne Modifier as a non-catalog request (Figure 5C). <\/p>\n<figure class=\"wp-block-image\"><img decoding=\"async\" src=\"https:\/\/www.idtdna.com\/page\/wp-content\/uploads\/idt-images\/cd255a15-3279-6e2e-aa53-ff00001c1b3c-postsynth-conj_fig-5.jpg\" data-displaymode=\"Original\" alt=\"alkyne modifiers that can be used in click chemistry\" title=\"D-OLI12PS-Postsynth conj-F5\" \/><figcaption class=\"image-caption\">Figure 5. Alkyne modifiers. These molecules are used to introduce an alkyne group within the oligonucleotide that can be used in a click reaction to conjugate the modification of one&rsquo;s choice.<\/figcaption><\/figure>\n<\/p>\n<h2>IDT NHS ester and click chemistry modifications<\/h2>\n<p>Table 1 shows current IDT catalog offerings of NHS ester and click chemistry modifications. Don't see the modification you need in our catalog? No worries. IDT routinely accepts requests for custom oligo modifications outside of our normal catalog offerings. Simply <a href=\"\/pages\/about\/contact-us\">contact us<\/a>&nbsp;to request non-catalog products or if you have questions about oligonucleotide modifications. <strong>Table 1. NHS ester modifications.<\/strong><\/p>\n<table class=\"table table-condensed\">\n<tbody>\n<tr>\n<td><strong>Modification name<\/strong><\/td>\n<td><strong>5'<\/strong><\/td>\n<td><strong>Internal<\/strong><\/td>\n<td><strong>3'<\/strong><\/td>\n<\/tr>\n<tr>\n<td>6-FAM<\/td>\n<td>&bull;<\/td>\n<td> <\/td>\n<td>&bull;<\/td>\n<\/tr>\n<tr>\n<td>Alexa Fluor<sup>&reg;<\/sup> 488<\/td>\n<td>&bull;<\/td>\n<td> <\/td>\n<td>&bull;<\/td>\n<\/tr>\n<tr>\n<td>Alexa Fluor&nbsp;532<\/td>\n<td>&bull;<\/td>\n<td> <\/td>\n<td>&bull;<\/td>\n<\/tr>\n<tr>\n<td>Alexa Fluor&nbsp;546<\/td>\n<td>&bull;<\/td>\n<td> <\/td>\n<td>&bull;<\/td>\n<\/tr>\n<tr>\n<td>Alexa Fluor&nbsp;594<\/td>\n<td>&bull;<\/td>\n<td> <\/td>\n<td>&bull;<\/td>\n<\/tr>\n<tr>\n<td>Alexa Fluor&nbsp;647<\/td>\n<td>&bull;<\/td>\n<td> <\/td>\n<td>&bull;<\/td>\n<\/tr>\n<tr>\n<td>Alexa Fluor&nbsp;660<\/td>\n<td>&bull;<\/td>\n<td> <\/td>\n<td>&bull;<\/td>\n<\/tr>\n<tr>\n<td>Alexa Fluor&nbsp;750<\/td>\n<td>&bull;<\/td>\n<td> <\/td>\n<td>&bull;<\/td>\n<\/tr>\n<tr>\n<td>ATTO&trade; 488<\/td>\n<td>&bull;<\/td>\n<td> <\/td>\n<td>&bull;<\/td>\n<\/tr>\n<tr>\n<td>ATTO 532<\/td>\n<td>&bull;<\/td>\n<td> <\/td>\n<td>&bull;<\/td>\n<\/tr>\n<tr>\n<td>ATTO 550<\/td>\n<td>&bull;<\/td>\n<td> <\/td>\n<td>&bull;<\/td>\n<\/tr>\n<tr>\n<td>ATTO 565<\/td>\n<td>&bull;<\/td>\n<td> <\/td>\n<td>&bull;<\/td>\n<\/tr>\n<tr>\n<td>ATTO Rho101<\/td>\n<td>&bull;<\/td>\n<td> <\/td>\n<td>&bull;<\/td>\n<\/tr>\n<tr>\n<td>ATTO 590<\/td>\n<td>&bull;<\/td>\n<td> <\/td>\n<td>&bull;<\/td>\n<\/tr>\n<tr>\n<td>ATTO 633<\/td>\n<td>&bull;<\/td>\n<td> <\/td>\n<td>&bull;<\/td>\n<\/tr>\n<tr>\n<td>ATTO 647N<\/td>\n<td>&bull;<\/td>\n<td> <\/td>\n<td>&bull;<\/td>\n<\/tr>\n<tr>\n<td>Azide (NHS Ester)<\/td>\n<td>&bull;<\/td>\n<td>&bull;<\/td>\n<td>&bull;<\/td>\n<\/tr>\n<tr>\n<td>Digoxigenin<\/td>\n<td>&bull;<\/td>\n<td> <\/td>\n<td>&bull;<\/td>\n<\/tr>\n<tr>\n<td>Dy 750&trade;<\/td>\n<td>&bull;<\/td>\n<td> <\/td>\n<td> <\/td>\n<\/tr>\n<tr>\n<td>IRDye<sup>&reg;<\/sup> 800<\/td>\n<td>&bull;<\/td>\n<td> <\/td>\n<td> <\/td>\n<\/tr>\n<tr>\n<td>JOE<\/td>\n<td>&bull;<\/td>\n<td> <\/td>\n<td>&bull;<\/td>\n<\/tr>\n<tr>\n<td>LightCycler<sup>&reg;<\/sup> 640<\/td>\n<td>&bull;<\/td>\n<td> <\/td>\n<td>&bull;<\/td>\n<\/tr>\n<tr>\n<td>MAX<\/td>\n<td>&bull;<\/td>\n<td> <\/td>\n<td>&bull;<\/td>\n<\/tr>\n<tr>\n<td>Rhodamine Green-X&trade;<\/td>\n<td>&bull;<\/td>\n<td> <\/td>\n<td>&bull;<\/td>\n<\/tr>\n<tr>\n<td>Rhodamine Red-X<\/td>\n<td>&bull;<\/td>\n<td> <\/td>\n<td>&bull;<\/td>\n<\/tr>\n<tr>\n<td>ROX<\/td>\n<td>&bull;<\/td>\n<td> <\/td>\n<td>&bull;<\/td>\n<\/tr>\n<tr>\n<td>TAMRA<\/td>\n<td>&bull;<\/td>\n<td>&bull;<\/td>\n<td>&bull;<\/td>\n<\/tr>\n<tr>\n<td>Texas Red-X<\/td>\n<td>&bull;<\/td>\n<td> <\/td>\n<td>&bull;<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"excerpt":{"rendered":"<p>Phosphoramidite synthesis vs. post-synthesis conjugation Modifications can be incorporated into synthetic oligonucleotides in a variety of ways. Standard DNA bases are synthetically coupled via phosphoramidite chemistry. The reaction proceeds in the 3' to 5' direction where the 5' hydroxyl group of each base attaches to the 3' phosphate group of the next base. Many modifications, [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"ct_builder_shortcodes":"","ct_template_type":"","ct_parent_template":0,"inline_featured_image":false,"footnotes":""},"class_list":["post-813","post","type-post","status-publish","format-standard","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.0 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Oligo modification\u2014Post-synthesis conjugation | IDT<\/title>\n<meta name=\"description\" content=\"Learn how IDT can add modifications to your oligos during phosphoramidite synthesis or modify oligos post-synthesis with NHS ester chemistry or click chemistry\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" 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