{"id":827,"date":"2015-12-29T06:00:00","date_gmt":"2015-12-29T06:00:00","guid":{"rendered":"https:\/\/www.idtdna.com\/page\/increase-the-tm-of-short-at-rich-primers-and-probes"},"modified":"2025-08-22T16:07:40","modified_gmt":"2025-08-22T16:07:40","slug":"increase-the-tm-of-short-at-rich-primers-and-probes","status":"publish","type":"post","link":"https:\/\/www.idtdna.com\/page\/support-and-education\/decoded-plus\/increase-the-tm-of-short-at-rich-primers-and-probes\/","title":{"rendered":"Increase the melting temperature of short, AT-rich primers and probes"},"content":{"rendered":"

Quick facts <\/h3>\n

Availability: <\/strong>DNA and RNA Location:<\/strong> 5', internal, and 3' Scales:<\/strong> 100 nmol to large scale Purification:<\/strong> Standard desalt or HPLC<\/a> Ordering (modification codes):<\/strong> 5Super-dT\/, \/iSuper-dT\/, \/3Super-dT\/<\/p>\n

Super T (5-hydroxybutynyl-2'-deoxyuridine; Figure 1) is a modified dT base that can be included in an oligonucleotide primer and probe sequences to stabilize their hybridization [1,2<\/a>]. Super T base-pairing with dA during primer or probe hybridization provides a ~2°C increase in melting temperature (Tm<\/span><\/sub>) per Super T insertion [1<\/a>]. This Tm<\/span><\/sub> increase can be important when working with short primers or probes that are AT-rich.<\/p>\n

The Super T modified base is extended normally by polymerases, including Taq polymerase.<\/p>\n

\"Structure
Figure 1. Structure of 3′–3′ or 5′–5′ reversed linkages.<\/figcaption><\/figure>\n<\/p>\n

How to order<\/h3>\n

To include Super T bases within your oligo, enter the IDT ordering symbol (\/5Super-dT\/<\/a><\/strong>, \/iSuper-dT\/<\/a><\/strong>, or \/3Super-dT\/<\/a><\/strong>) in the desired location within your sequence. You can request Super T modified oligonucleotides with standard desalt or HPLC purification.<\/p>\n","protected":false},"excerpt":{"rendered":"

Quick facts Availability: DNA and RNA Location: 5', internal, and 3' Scales: 100 nmol to large scale Purification: Standard desalt or HPLC Ordering (modification codes): 5Super-dT\/, \/iSuper-dT\/, \/3Super-dT\/ Super T (5-hydroxybutynyl-2'-deoxyuridine; Figure 1) is a modified dT base that can be included in an oligonucleotide primer and probe sequences to stabilize their hybridization [1,2]. Super […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"ct_builder_shortcodes":"","ct_template_type":"","ct_parent_template":0,"inline_featured_image":false,"footnotes":""},"class_list":["post-827","post","type-post","status-publish","format-standard","hentry"],"acf":[],"yoast_head":"\nIncrease the melting temperature of short, AT-rich primers and probes | IDT<\/title>\n<meta name=\"description\" content=\"Add this modified base to increase the melting temperature (Tm) of primers and probes. 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