{"id":837,"date":"2013-01-18T06:00:00","date_gmt":"2013-01-18T06:00:00","guid":{"rendered":"https:\/\/www.idtdna.com\/page\/new-alternative-dyes-for-fish-fret-and-qpcr"},"modified":"2025-08-22T16:07:48","modified_gmt":"2025-08-22T16:07:48","slug":"new-alternative-dyes-for-fish-fret-and-qpcr","status":"publish","type":"post","link":"https:\/\/www.idtdna.com\/page\/support-and-education\/decoded-plus\/new-alternative-dyes-for-fish-fret-and-qpcr\/","title":{"rendered":"Alternative dyes for FISH, FRET, and qPCR"},"content":{"rendered":"

ATTO-labeled oligonucleotides offer additional alternatives for applications including fluorescence in situ hybridization (FISH), fluorescence resonance energy transfer (FRET), and dual-labeled probes used in qPCR experiments.* ATTO dyes are a group of patented fluorescent markers that feature strong absorption, photostability, and fluorescence quantum yield. <\/p>\n

The ATTO dyes are perfect substitutes for dyes such as ROX™, VIC®<\/sup>, LIZ®<\/sup>, and Alexa Fluor®<\/sup> dyes. For qPCR applications, dye calibration will be needed for each qPCR instrument [1<\/a>].<\/p>\n

Ordering ATTO dyes<\/h2>\n

Catalog ATTO dyes can be attached to 100 nmol, 250 nmol, and 1 μmol DNA and RNA oligonucleotides, or duplex products. To order, visit the DNA Oligos<\/a> or RNA Oligos<\/a> ordering pages, where you can choose an ATTO dye as either a 5′ or 3′ modification. All ATTO-labeled oligos from IDT will be HPLC-purified. <\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
\n

Catalog ATTO dye<\/h3>\n<\/th>\n

\n

Alternative for:<\/h3>\n<\/th>\n<\/tr>\n<\/thead>\n

5' or 3' ATTO 488 (NHS Ester)<\/td>\nAlexa Fluor 488<\/td>\n<\/tr>\n
5' or 3' ATTO 532 (NHS Ester)<\/td>\nVIC<\/td>\n<\/tr>\n
5' or 3' ATTO 550 (NHS Ester)<\/td>\nNED™ and Alexa Fluor 555<\/td>\n<\/tr>\n
5' or 3' ATTO 565 (NHS Ester)<\/td>\nPET™<\/td>\n<\/tr>\n
5' or 3' ATTO Rho101 (NHS Ester)<\/td>\nROX™<\/td>\n<\/tr>\n
5' or 3' ATTO 590 (NHS Ester)<\/td>\nAlexa Fluor 594<\/td>\n<\/tr>\n
5' or 3' ATTO 633 (NHS Ester)<\/td>\nLIZ<\/td>\n<\/tr>\n
5' or 3' ATTO 647N (NHS Ester)<\/td>\nAlexa Fluor 647<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

For questions about ATTO dye modifications or products not found on this list, including internal dye placement or dual-labeled probes, reach out at contact<\/a>us<\/a>.<\/p>\n

\"FOF-ATTO
Figure 1. Strong fluorescence of ATTO dyes. Rat stomach stained with mouse anti-smooth muscle α-actin antibody followed by ATTO 488 anti-mouse IgG (green); and polyclonal rabbit anti-cytokeratin followed by ATTO 647N anti-rabbit IgG (red) [Image courtesy of ATTO-TEC GmbH]. For detailed information on these (and other) ATTO dyes, visit ATTO-TEC.<\/figcaption><\/figure>\n

*ATTO dyes have not been tested on Illumina®<\/sup> platforms for use with certain fluorescent applications, such as sequencing, genotyping, and fragment analysis. Researchers must perform independent testing for such use.<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

ATTO-labeled oligonucleotides offer additional alternatives for applications including fluorescence in situ hybridization (FISH), fluorescence resonance energy transfer (FRET), and dual-labeled probes used in qPCR experiments.* ATTO dyes are a group of patented fluorescent markers that feature strong absorption, photostability, and fluorescence quantum yield. The ATTO dyes are perfect substitutes for dyes such as ROX™, VIC®, […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"ct_builder_shortcodes":"","ct_template_type":"","ct_parent_template":0,"inline_featured_image":false,"footnotes":""},"class_list":["post-837","post","type-post","status-publish","format-standard","hentry"],"acf":[],"yoast_head":"\nAlternative dyes for FISH, FRET, and qPCR | IDT<\/title>\n<meta name=\"description\" content=\"Use ATTO-labeled oligonucleotides as alternatives in applications including FISH, FRET, and dual-labeled probes for qPCR experiments.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.idtdna.com\/page\/support-and-education\/decoded-plus\/new-alternative-dyes-for-fish-fret-and-qpcr\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Alternative dyes for FISH, FRET, and qPCR | IDT\" \/>\n<meta property=\"og:description\" content=\"Use ATTO-labeled oligonucleotides as alternatives in applications including FISH, FRET, and dual-labeled probes for qPCR experiments.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.idtdna.com\/page\/support-and-education\/decoded-plus\/new-alternative-dyes-for-fish-fret-and-qpcr\/\" \/>\n<meta property=\"og:site_name\" content=\"IDT\" \/>\n<meta property=\"article:published_time\" content=\"2013-01-18T06:00:00+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2025-08-22T16:07:48+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/sfvideo.blob.core.windows.net\/sitefinity\/images\/default-source\/default-album\/decoded-temp-image-storage\/fof-atto-dyes-fig-1.bmp?sfvrsn=743f3607_14\" \/>\n<meta name=\"author\" content=\"supreme_admin\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"supreme_admin\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"2 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\/\/www.idtdna.com\/page\/support-and-education\/decoded-plus\/new-alternative-dyes-for-fish-fret-and-qpcr\/#article\",\"isPartOf\":{\"@id\":\"https:\/\/www.idtdna.com\/page\/support-and-education\/decoded-plus\/new-alternative-dyes-for-fish-fret-and-qpcr\/\"},\"author\":{\"name\":\"supreme_admin\",\"@id\":\"https:\/\/www.idtdna.com\/page\/#\/schema\/person\/5259624c7d0e737830199587f61fd4c0\"},\"headline\":\"Alternative dyes for FISH, FRET, and qPCR\",\"datePublished\":\"2013-01-18T06:00:00+00:00\",\"dateModified\":\"2025-08-22T16:07:48+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\/\/www.idtdna.com\/page\/support-and-education\/decoded-plus\/new-alternative-dyes-for-fish-fret-and-qpcr\/\"},\"wordCount\":302,\"commentCount\":0,\"image\":{\"@id\":\"https:\/\/www.idtdna.com\/page\/support-and-education\/decoded-plus\/new-alternative-dyes-for-fish-fret-and-qpcr\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/sfvideo.blob.core.windows.net\/sitefinity\/images\/default-source\/default-album\/decoded-temp-image-storage\/fof-atto-dyes-fig-1.bmp?sfvrsn=743f3607_14\",\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"CommentAction\",\"name\":\"Comment\",\"target\":[\"https:\/\/www.idtdna.com\/page\/support-and-education\/decoded-plus\/new-alternative-dyes-for-fish-fret-and-qpcr\/#respond\"]}]},{\"@type\":\"WebPage\",\"@id\":\"https:\/\/www.idtdna.com\/page\/support-and-education\/decoded-plus\/new-alternative-dyes-for-fish-fret-and-qpcr\/\",\"url\":\"https:\/\/www.idtdna.com\/page\/support-and-education\/decoded-plus\/new-alternative-dyes-for-fish-fret-and-qpcr\/\",\"name\":\"Alternative dyes for FISH, FRET, and qPCR | IDT\",\"isPartOf\":{\"@id\":\"https:\/\/www.idtdna.com\/page\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\/\/www.idtdna.com\/page\/support-and-education\/decoded-plus\/new-alternative-dyes-for-fish-fret-and-qpcr\/#primaryimage\"},\"image\":{\"@id\":\"https:\/\/www.idtdna.com\/page\/support-and-education\/decoded-plus\/new-alternative-dyes-for-fish-fret-and-qpcr\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/sfvideo.blob.core.windows.net\/sitefinity\/images\/default-source\/default-album\/decoded-temp-image-storage\/fof-atto-dyes-fig-1.bmp?sfvrsn=743f3607_14\",\"datePublished\":\"2013-01-18T06:00:00+00:00\",\"dateModified\":\"2025-08-22T16:07:48+00:00\",\"author\":{\"@id\":\"https:\/\/www.idtdna.com\/page\/#\/schema\/person\/5259624c7d0e737830199587f61fd4c0\"},\"description\":\"Use ATTO-labeled oligonucleotides as alternatives in applications including FISH, FRET, and dual-labeled probes for qPCR experiments.\",\"breadcrumb\":{\"@id\":\"https:\/\/www.idtdna.com\/page\/support-and-education\/decoded-plus\/new-alternative-dyes-for-fish-fret-and-qpcr\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/www.idtdna.com\/page\/support-and-education\/decoded-plus\/new-alternative-dyes-for-fish-fret-and-qpcr\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/www.idtdna.com\/page\/support-and-education\/decoded-plus\/new-alternative-dyes-for-fish-fret-and-qpcr\/#primaryimage\",\"url\":\"https:\/\/sfvideo.blob.core.windows.net\/sitefinity\/images\/default-source\/default-album\/decoded-temp-image-storage\/fof-atto-dyes-fig-1.bmp?sfvrsn=743f3607_14\",\"contentUrl\":\"https:\/\/sfvideo.blob.core.windows.net\/sitefinity\/images\/default-source\/default-album\/decoded-temp-image-storage\/fof-atto-dyes-fig-1.bmp?sfvrsn=743f3607_14\"},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/www.idtdna.com\/page\/support-and-education\/decoded-plus\/new-alternative-dyes-for-fish-fret-and-qpcr\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\/\/www.idtdna.com\/page\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Alternative dyes for FISH, FRET, and qPCR\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/www.idtdna.com\/page\/#website\",\"url\":\"https:\/\/www.idtdna.com\/page\/\",\"name\":\"Integrated DNA Technologies\",\"description\":\"\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/www.idtdna.com\/page\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"},{\"@type\":\"Person\",\"@id\":\"https:\/\/www.idtdna.com\/page\/#\/schema\/person\/5259624c7d0e737830199587f61fd4c0\",\"name\":\"supreme_admin\",\"image\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/www.idtdna.com\/page\/#\/schema\/person\/image\/\",\"url\":\"https:\/\/secure.gravatar.com\/avatar\/b33db2b8496475f7b5b0a33de06c5416bdad923515045a5e9106efc71f7916cc?s=96&d=mm&r=g\",\"contentUrl\":\"https:\/\/secure.gravatar.com\/avatar\/b33db2b8496475f7b5b0a33de06c5416bdad923515045a5e9106efc71f7916cc?s=96&d=mm&r=g\",\"caption\":\"supreme_admin\"},\"sameAs\":[\"https:\/\/stage.idtdna.com\/page\"]}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Alternative dyes for FISH, FRET, and qPCR | IDT","description":"Use ATTO-labeled oligonucleotides as alternatives in applications including FISH, FRET, and dual-labeled probes for qPCR experiments.","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.idtdna.com\/page\/support-and-education\/decoded-plus\/new-alternative-dyes-for-fish-fret-and-qpcr","og_locale":"en_US","og_type":"article","og_title":"Alternative dyes for FISH, FRET, and qPCR | IDT","og_description":"Use ATTO-labeled oligonucleotides as alternatives in applications including FISH, FRET, and dual-labeled probes for qPCR experiments.","og_url":"https:\/\/www.idtdna.com\/page\/support-and-education\/decoded-plus\/new-alternative-dyes-for-fish-fret-and-qpcr\/","og_site_name":"IDT","article_published_time":"2013-01-18T06:00:00+00:00","article_modified_time":"2025-08-22T16:07:48+00:00","og_image":[{"url":"https:\/\/sfvideo.blob.core.windows.net\/sitefinity\/images\/default-source\/default-album\/decoded-temp-image-storage\/fof-atto-dyes-fig-1.bmp?sfvrsn=743f3607_14","type":"","width":"","height":""}],"author":"supreme_admin","twitter_card":"summary_large_image","twitter_misc":{"Written by":"supreme_admin","Est. reading time":"2 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"Article","@id":"https:\/\/www.idtdna.com\/page\/support-and-education\/decoded-plus\/new-alternative-dyes-for-fish-fret-and-qpcr\/#article","isPartOf":{"@id":"https:\/\/www.idtdna.com\/page\/support-and-education\/decoded-plus\/new-alternative-dyes-for-fish-fret-and-qpcr\/"},"author":{"name":"supreme_admin","@id":"https:\/\/www.idtdna.com\/page\/#\/schema\/person\/5259624c7d0e737830199587f61fd4c0"},"headline":"Alternative dyes for FISH, FRET, and qPCR","datePublished":"2013-01-18T06:00:00+00:00","dateModified":"2025-08-22T16:07:48+00:00","mainEntityOfPage":{"@id":"https:\/\/www.idtdna.com\/page\/support-and-education\/decoded-plus\/new-alternative-dyes-for-fish-fret-and-qpcr\/"},"wordCount":302,"commentCount":0,"image":{"@id":"https:\/\/www.idtdna.com\/page\/support-and-education\/decoded-plus\/new-alternative-dyes-for-fish-fret-and-qpcr\/#primaryimage"},"thumbnailUrl":"https:\/\/sfvideo.blob.core.windows.net\/sitefinity\/images\/default-source\/default-album\/decoded-temp-image-storage\/fof-atto-dyes-fig-1.bmp?sfvrsn=743f3607_14","inLanguage":"en-US","potentialAction":[{"@type":"CommentAction","name":"Comment","target":["https:\/\/www.idtdna.com\/page\/support-and-education\/decoded-plus\/new-alternative-dyes-for-fish-fret-and-qpcr\/#respond"]}]},{"@type":"WebPage","@id":"https:\/\/www.idtdna.com\/page\/support-and-education\/decoded-plus\/new-alternative-dyes-for-fish-fret-and-qpcr\/","url":"https:\/\/www.idtdna.com\/page\/support-and-education\/decoded-plus\/new-alternative-dyes-for-fish-fret-and-qpcr\/","name":"Alternative dyes for FISH, FRET, and qPCR | IDT","isPartOf":{"@id":"https:\/\/www.idtdna.com\/page\/#website"},"primaryImageOfPage":{"@id":"https:\/\/www.idtdna.com\/page\/support-and-education\/decoded-plus\/new-alternative-dyes-for-fish-fret-and-qpcr\/#primaryimage"},"image":{"@id":"https:\/\/www.idtdna.com\/page\/support-and-education\/decoded-plus\/new-alternative-dyes-for-fish-fret-and-qpcr\/#primaryimage"},"thumbnailUrl":"https:\/\/sfvideo.blob.core.windows.net\/sitefinity\/images\/default-source\/default-album\/decoded-temp-image-storage\/fof-atto-dyes-fig-1.bmp?sfvrsn=743f3607_14","datePublished":"2013-01-18T06:00:00+00:00","dateModified":"2025-08-22T16:07:48+00:00","author":{"@id":"https:\/\/www.idtdna.com\/page\/#\/schema\/person\/5259624c7d0e737830199587f61fd4c0"},"description":"Use ATTO-labeled oligonucleotides as alternatives in applications including FISH, FRET, and dual-labeled probes for qPCR experiments.","breadcrumb":{"@id":"https:\/\/www.idtdna.com\/page\/support-and-education\/decoded-plus\/new-alternative-dyes-for-fish-fret-and-qpcr\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/www.idtdna.com\/page\/support-and-education\/decoded-plus\/new-alternative-dyes-for-fish-fret-and-qpcr\/"]}]},{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/www.idtdna.com\/page\/support-and-education\/decoded-plus\/new-alternative-dyes-for-fish-fret-and-qpcr\/#primaryimage","url":"https:\/\/sfvideo.blob.core.windows.net\/sitefinity\/images\/default-source\/default-album\/decoded-temp-image-storage\/fof-atto-dyes-fig-1.bmp?sfvrsn=743f3607_14","contentUrl":"https:\/\/sfvideo.blob.core.windows.net\/sitefinity\/images\/default-source\/default-album\/decoded-temp-image-storage\/fof-atto-dyes-fig-1.bmp?sfvrsn=743f3607_14"},{"@type":"BreadcrumbList","@id":"https:\/\/www.idtdna.com\/page\/support-and-education\/decoded-plus\/new-alternative-dyes-for-fish-fret-and-qpcr\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/www.idtdna.com\/page\/"},{"@type":"ListItem","position":2,"name":"Alternative dyes for FISH, FRET, and qPCR"}]},{"@type":"WebSite","@id":"https:\/\/www.idtdna.com\/page\/#website","url":"https:\/\/www.idtdna.com\/page\/","name":"Integrated DNA Technologies","description":"","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/www.idtdna.com\/page\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"},{"@type":"Person","@id":"https:\/\/www.idtdna.com\/page\/#\/schema\/person\/5259624c7d0e737830199587f61fd4c0","name":"supreme_admin","image":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/www.idtdna.com\/page\/#\/schema\/person\/image\/","url":"https:\/\/secure.gravatar.com\/avatar\/b33db2b8496475f7b5b0a33de06c5416bdad923515045a5e9106efc71f7916cc?s=96&d=mm&r=g","contentUrl":"https:\/\/secure.gravatar.com\/avatar\/b33db2b8496475f7b5b0a33de06c5416bdad923515045a5e9106efc71f7916cc?s=96&d=mm&r=g","caption":"supreme_admin"},"sameAs":["https:\/\/stage.idtdna.com\/page"]}]}},"_links":{"self":[{"href":"https:\/\/www.idtdna.com\/page\/wp-json\/wp\/v2\/posts\/837","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.idtdna.com\/page\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.idtdna.com\/page\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.idtdna.com\/page\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.idtdna.com\/page\/wp-json\/wp\/v2\/comments?post=837"}],"version-history":[{"count":1,"href":"https:\/\/www.idtdna.com\/page\/wp-json\/wp\/v2\/posts\/837\/revisions"}],"predecessor-version":[{"id":911,"href":"https:\/\/www.idtdna.com\/page\/wp-json\/wp\/v2\/posts\/837\/revisions\/911"}],"wp:attachment":[{"href":"https:\/\/www.idtdna.com\/page\/wp-json\/wp\/v2\/media?parent=837"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}