{"id":903,"date":"2012-04-11T20:02:00","date_gmt":"2012-04-11T20:02:00","guid":{"rendered":"https:\/\/www.idtdna.com\/page\/new-5-hydroxymethyl-dc"},"modified":"2025-08-22T16:10:30","modified_gmt":"2025-08-22T16:10:30","slug":"new-5-hydroxymethyl-dc","status":"publish","type":"post","link":"https:\/\/www.idtdna.com\/page\/support-and-education\/decoded-plus\/new-5-hydroxymethyl-dc\/","title":{"rendered":"5-Hydroxymethyl-dC for epigenetic research"},"content":{"rendered":"<h3>Quick fac<strong>ts: <\/strong><\/h3>\n<p><strong>Availability:<\/strong> DNA <strong>Location:<\/strong> 5&prime;, 3&prime;, or internal <strong>Scale: <\/strong>100 nmol, 250 nmol, 1 &micro;mol, 5 &micro;mol, and 10 &micro;mol<strong> Purification:&nbsp;<\/strong>HPLC required <strong>Ordering symbols: <\/strong> &nbsp;&nbsp;&nbsp;&nbsp; For 5&prime; placement, use \/55HydMe-dC\/ &nbsp;&nbsp;&nbsp;&nbsp; For 3&prime; placement, use \/35HydMe-dC\/ &nbsp;&nbsp;&nbsp;&nbsp; For internal placement, use \/i5HydMe-dC\/<\/p>\n<p><img decoding=\"async\" src=\"https:\/\/www.idtdna.com\/page\/wp-content\/uploads\/idt-images\/73895d15-3279-6e2e-aa53-ff00001c1b3c-fof-5-hydroxymethyl-dc_structure.png\" data-displaymode=\"Original\" alt=\"FOF - 5-Hydroxymethyl-dC_structure\" title=\"D-OLI12MH-Hydroxymethyl-dC-F1\" \/><\/p>\n<p>The methylation of deoxycytosine (dC) to 5-methylcytosine (5-mC) has long been studied for the important role it plays as an epigenetic marker in gene expression and regulation. Now researchers are turning their attention to 5-hydroxymethylcytosine (5-hmC), a recently discovered &ldquo;sixth base&rdquo; [1]. In mammalian cells, 5-hmC has been detected in all tissue types, with the highest levels observed in the brain and spinal cord [2,3]. Jin, Wu, et al. [4] were among the first to compare levels and locations of 5-hmC to 5-mC in human brain tissue, and reported its enrichment at promoters and in intragenic regions. It has been reported that 5-mC is converted to 5-hmC by TET proteins, which perform various roles in gene expression. While the exact biological function of 5-hmC is unknown, it has been hypothesized that it may be an intermediate in a demethylation pathway or may act as an additional epigenetic factor [5].<\/p>\n<h3>Ordering oligos with 5-Hydroxymethyl-dC<\/h3>\n<p>You can order oligos with this modification introduced at any position using standard. To order an oligonucleotide modified with 5-hydroxymethylcytosine: select 5-Hydroxymethyl-dC from the modifications tab on the oligonucleotide order page. Alternatively, use the appropriate modification code. <\/p>\n<h3>Other modifications to facilitate your research<\/h3>\n<p>See what other modifications are available from IDT. You can find a list of standard modifications we make to oligonucleotides on the <a href=\"\/pages\/products\/custom-dna-rna\/oligo-modifications\">Modifications page <\/a>of our online catalog. And if you don&rsquo;t find what you are looking for, just send us a request. We often synthesize special, non-catalog requests. Contact us at <a href=\"mailto:noncat@idtdna.com\">noncat@idtdna.com<\/a>.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Quick facts: Availability: DNA Location: 5&prime;, 3&prime;, or internal Scale: 100 nmol, 250 nmol, 1 &micro;mol, 5 &micro;mol, and 10 &micro;mol Purification:&nbsp;HPLC required Ordering symbols: &nbsp;&nbsp;&nbsp;&nbsp; For 5&prime; placement, use \/55HydMe-dC\/ &nbsp;&nbsp;&nbsp;&nbsp; For 3&prime; placement, use \/35HydMe-dC\/ &nbsp;&nbsp;&nbsp;&nbsp; For internal placement, use \/i5HydMe-dC\/ The methylation of deoxycytosine (dC) to 5-methylcytosine (5-mC) has long been studied [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"ct_builder_shortcodes":"","ct_template_type":"","ct_parent_template":0,"inline_featured_image":false,"footnotes":""},"class_list":["post-903","post","type-post","status-publish","format-standard","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.0 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>5-Hydroxymethyl-dC for epigenetic research - IDT<\/title>\n<meta name=\"description\" content=\"Naturally occurring 5-hydroxymethylcytosine (5-hmC) has been hypothesized to be an intermediate in a demethylation pathway or an additional epigenetic factor. 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