{"id":907,"date":"2015-08-27T15:55:00","date_gmt":"2015-08-27T15:55:00","guid":{"rendered":"https:\/\/www.idtdna.com\/page\/modification-highlight-super-g-8-aza-7-deazaguanosine"},"modified":"2025-08-22T16:10:33","modified_gmt":"2025-08-22T16:10:33","slug":"modification-highlight-super-g-8-aza-7-deazaguanosine","status":"publish","type":"post","link":"https:\/\/www.idtdna.com\/page\/support-and-education\/decoded-plus\/modification-highlight-super-g-8-aza-7-deazaguanosine\/","title":{"rendered":"Design functional oligos with poly G runs"},"content":{"rendered":"

Quick facts:<\/h3>\n

Availability: <\/strong>DNA and RNA Location:<\/strong> 5′, internal, and 3′ Synthesis scale:<\/strong> 100 nmol to large scale Purification options:<\/strong> Standard desalt or HPLC IDT ordering symbol:<\/strong> \/5Super-dG\/<\/a>, \/iSuper-dG\/<\/a>, \/3Super-dG\/<\/a><\/p>\n

Need an oligo with a poly G run?<\/strong> <\/h2>\n

When trying to order an oligo with several sequential guanine residues, you will often receive a warning to the effect: <\/p>\n

“…Runs of 4 or more G bases can result in a cruciform structure, also known as a guanine tetraplex that may interfere with downstream applications. In addition, oligo sequences containing runs of 6 or more G bases can be difficult to manufacture…”<\/em> <\/p>\n

Super G (8-aza-7-deaza dG) bases (Figure 1) are pyrazolo[3,4-d]pyrimidine analogs of guanine that eliminate naturally occurring, non-Watson-and-Crick secondary structures associated with guanine-rich sequences [1].<\/p>\n

\"art84-MH-SuperG-Fig1\"
Figure 1. Super G (8-aza-7-deaza dG) modified base.<\/figcaption><\/figure>\n<\/p>\n

Including a Super G modified base in place of one of a series of G residues in an oligo sequence: <\/p>\n