{"id":936,"date":"2013-10-21T18:42:00","date_gmt":"2013-10-21T18:42:00","guid":{"rendered":"https:\/\/www.idtdna.com\/page\/a-high-throughput-high-resolution-melting-protocol-for-identifying-single-nucleotide-polymorphisms"},"modified":"2025-08-22T16:10:51","modified_gmt":"2025-08-22T16:10:51","slug":"a-high-throughput-high-resolution-melting-protocol-for-identifying-single-nucleotide-polymorphisms","status":"publish","type":"post","link":"https:\/\/www.idtdna.com\/page\/support-and-education\/decoded-plus\/a-high-throughput-high-resolution-melting-protocol-for-identifying-single-nucleotide-polymorphisms\/","title":{"rendered":"A high-throughput, high-resolution melting protocol for identifying SNPs"},"content":{"rendered":"<p>Single nucleotide polymorphisms (SNPs) are commonly studied for their contribution to genetic variation and disease. In this article, Bruzzone et al. present a novel method for evaluating SNPs in a fast, high-throughput protocol. The protocol relies on careful primer design, and a &ldquo;cloning fragment length double-stranded DNA control template&rdquo; to verify the identity of homozygous peaks in the melt-curve analysis for SNP detection. Sequence-verified <a href=\"\/pages\/products\/genes-and-gene-fragments\/double-stranded-dna-fragments\/gblocks-gene-fragments\">gBlocks<sup>&reg;<\/sup> Gene Fragments<\/a>&nbsp;were an ideal solution for the melt-curve controls in this study, and were central to the SNP analysis.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Single nucleotide polymorphisms (SNPs) are commonly studied for their contribution to genetic variation and disease. In this article, Bruzzone et al. present a novel method for evaluating SNPs in a fast, high-throughput protocol. The protocol relies on careful primer design, and a &ldquo;cloning fragment length double-stranded DNA control template&rdquo; to verify the identity of homozygous [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"ct_builder_shortcodes":"","ct_template_type":"","ct_parent_template":0,"inline_featured_image":false,"footnotes":""},"class_list":["post-936","post","type-post","status-publish","format-standard","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.0 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>A high-throughput, high-resolution melting protocol for identifying SNPs - IDT<\/title>\n<meta name=\"description\" content=\"Protocol for rapid SNP evaluation by HTP melt-curve analysis.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.idtdna.com\/page\/support-and-education\/decoded-plus\/a-high-throughput-high-resolution-melting-protocol-for-identifying-single-nucleotide-polymorphisms\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"A high-throughput, high-resolution melting protocol for identifying SNPs - IDT\" \/>\n<meta property=\"og:description\" content=\"Protocol for rapid SNP evaluation by HTP melt-curve analysis.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/eu.idtdna.com\/page\/support-and-education\/decoded-plus\/a-high-throughput-high-resolution-melting-protocol-for-identifying-single-nucleotide-polymorphisms\/\" \/>\n<meta property=\"og:site_name\" content=\"IDT\" \/>\n<meta property=\"article:published_time\" content=\"2013-10-21T18:42:00+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2025-08-22T16:10:51+00:00\" \/>\n<meta name=\"author\" content=\"supreme_admin\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"supreme_admin\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\/\/eu.idtdna.com\/page\/support-and-education\/decoded-plus\/a-high-throughput-high-resolution-melting-protocol-for-identifying-single-nucleotide-polymorphisms\/#article\",\"isPartOf\":{\"@id\":\"https:\/\/eu.idtdna.com\/page\/support-and-education\/decoded-plus\/a-high-throughput-high-resolution-melting-protocol-for-identifying-single-nucleotide-polymorphisms\/\"},\"author\":{\"name\":\"supreme_admin\",\"@id\":\"https:\/\/www.idtdna.com\/page\/#\/schema\/person\/5259624c7d0e737830199587f61fd4c0\"},\"headline\":\"A high-throughput, high-resolution melting protocol for identifying SNPs\",\"datePublished\":\"2013-10-21T18:42:00+00:00\",\"dateModified\":\"2025-08-22T16:10:51+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\/\/eu.idtdna.com\/page\/support-and-education\/decoded-plus\/a-high-throughput-high-resolution-melting-protocol-for-identifying-single-nucleotide-polymorphisms\/\"},\"wordCount\":97,\"commentCount\":0,\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"CommentAction\",\"name\":\"Comment\",\"target\":[\"https:\/\/eu.idtdna.com\/page\/support-and-education\/decoded-plus\/a-high-throughput-high-resolution-melting-protocol-for-identifying-single-nucleotide-polymorphisms\/#respond\"]}]},{\"@type\":\"WebPage\",\"@id\":\"https:\/\/eu.idtdna.com\/page\/support-and-education\/decoded-plus\/a-high-throughput-high-resolution-melting-protocol-for-identifying-single-nucleotide-polymorphisms\/\",\"url\":\"https:\/\/eu.idtdna.com\/page\/support-and-education\/decoded-plus\/a-high-throughput-high-resolution-melting-protocol-for-identifying-single-nucleotide-polymorphisms\/\",\"name\":\"A high-throughput, high-resolution melting protocol for identifying SNPs - 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