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Do synthetic oligonucleotides contain a 5’ phosphate?

The 5’ end of an unmodified synthetic oligonucleotide terminates in a hydroxyl group. To obtain an oligonucleotide with a 5’ phosphate, use the code /5Phos/ in your sequence when you place the order, or phosphorylate the oligonucleotide with a simple kinase reaction before use.