Your Questions For Our Resident Expert
Why do I receive less than the requested scale quantity for a synthesized oligonucleotide?
The scale of the oligonucleotide synthesis reaction is based solely on the starting material. As no chemical reaction is 100% efficient, the final deliverable amount is never equal to the starting amount for synthesis. Even simple DNA bases do not couple with 100% efficiency. As a result, the final synthesis amount is never equal to the starting synthesis scale. Modifications, secondary structures, and purification services further affect the final yield for a given construct. IDT offers guaranteed yields based on all of these factors that define the minimum amount of oligonucleotide you will receive for a given construct.