Your Questions For Our Resident Expert
Why should I sequence multiple colonies when cloning?
Sometimes researchers will pick just a colony or two for sequencing. If they find problems with their clone, they may conclude something must be wrong with their primers when they simply have picked a bad colony. IDT recommends sequencing at least 8–10 separate colonies to get a realistic representation of the clonal population and to provide a good chance of finding the correct sequence if one exists.