Your Questions For Our Resident Expert
Do modified oligos require special storage and resuspension?
Most modified oligos can be stored and resuspended just as unmodified oligos, with the exception of photocleavable or photolabile modifications such as Bromo-dU, PC Spacer, PC Biotin, and PC Linker. These should be kept away from light and are therefore shipped in amber tubes. Also, amine-modified oligos that will be used in NHS ester reactions should not be hydrated in buffers containing amines (such as TRIS), as the amines in the buffer will compete with the amine-modified oligo in the reaction. These amine-modified oligos can instead be resuspended in 0.1 mM EDTA or nuclease-free water and stored at -20°C.