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What dyes should I select for my qPCR assay?

The most important thing is to select dyes that are compatible with your machine. FAM is generally compatible with all machines. If you would like suggestions about which dye to use, contact Customer Care at custcare@idtdna.com with the name of your machine and we can make a recommendation. IDT also offers a free PrimeTime® qPCR Applications Guide that contains additional information on available dyes, and instrument compatibility.