Gene Fragments are delivered dried down, normalized to 200 ng, which should provide sufficient DNA for 2–4 cloning reactions according to most protocols. Due to the probability of the polymerase introducing random errors, we do not recommend amplification of gBlocks Gene Fragments. However, if you do need to amplify your gBlocks Gene Fragments to obtain more material than the 200 ng provided, using a high-fidelity polymerase will help prevent the introduction of errors. For additional information, a protocol for amplification using the Phusion®
DNA polymerase (NEB) can be downloaded at www.idtdna. com/gBlocks
, under the Support tab.