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Cited
Scientific Publications Referencing IDT Products

Zika virus: Advances in disease modeling and detection

IDT is supporting global research aimed at reducing the widespread effects of Zika. Learn about the virus, and read a summary of the latest developments.

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qPCR assays for optimized viral detection in clinical samples

Learn how ZEN™ Double-Quenched Probes were used in a unique qPCR experiment to help rapidly and accurately detect the highly variable norovirus in clinical samples.

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Enzyme-linked DNA aptamer assay provides advantages over standard immunoassays

Citation summary: Find out how this research team used candidate aptamers in an enzyme-linked aptamer sorbent assay (ELASA) to detect human insulin-like growth factor-I, a biomarker for recombinant human growth hormone, used in athletic doping.

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RNA-guided gene drives for inheritance bias in yeast: Safe, responsible genome editing

Citation summary: Read about how a group of researchers use gBlocks Gene Fragments and novel precautionary measures to responsibly investigate Cas9-based eukaryotic inheritance bias of gene drives.

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ve-SEQ: An improved approach to high throughput, whole genome viral sequencing

Citation summary: Read how scientists at the University of Oxford use xGen® Lockdown® Probes in ve-SEQ, a novel method for detecting and sequencing HCV genotypes.

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Improved pathogen detection by multiplex RT-qPCR

Citation summary: Learn how gBlocks Gene Fragments can help optimize multiplex qPCR for pathogen detection in human clinical samples.

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A novel non-enzymatic assay for SNP detection in cancer DNA

Citation summary: Learn how scientists use a long, biotinylated Ultramer® Oligo to capture specific gene fragments for the identification of SNPs with single-molecule resolution. Their method is not only cost-effective, but avoids enzyme-based technologies, such as PCR and NGS, that vary in fidelity and increase risk of introducing amplification bias.

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Next generation sequencing for risk assessment in acute myeloid leukemia

Citation summary: Use of next generation sequencing (NGS) techniques with patients diagnosed with AML to identify associations between specific mutations and disease outcome. These techniques were also used to track the elimination of leukemia-specific mutations in AML patients following chemotherapy. The IDT xGen® AML Cancer Panel v1.0 was used to target and capture 264 commonly mutated genes in AML.

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Expanded genome modification roles for CRISPR/Cas9 using Cas9-VPR and shortened sgRNAs

Citation summary: This paper describes how Cas9 enzyme function can be modulated to perform genome editing and gene regulation functions simultaneously, and how these activities can be used to construct complex genetic circuits. IDT gBlocks® Gene Fragments were used to assemble U6-driven sgRNA expression cassettes and a CRISPR-repressible promoter (CRP) library.

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3C-MTS technology identifies distant genomic interactions made by a cancer risk locus

Citation summary: Chromosome conformation capture-based multiple target sequencing (3C-MTS) technology is used to obtain a genome-wide view of regions that physically interact with the prostate cancer risk locus 8q24. This method combines a 3C assay with multi-target capture sequencing using xGen® Lockdown® Probes (IDT).

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Gene panels vs. gene-by-gene analysis for assessing disease risk

Citation summary: A comparison of NGS-based gene panel and traditional testing data for diagnostic use and disease risk assessment in hereditary breast and ovarian cancer. xGen Lockdown Probes (IDT) were used to rescue drop-out regions of SureSelect (Agilent) probe panels.

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Somatic mutations acquired in BRCA1 during embryonic development can cause early-onset breast cancer

Citation summary: Focused sequencing using target capture probes helps identify a post-fertilization mutation in BRCA1 that may predict risk of early-onset breast cancer.

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CRISPR + Gibson Assembly® approach addresses cloning limitations

Citation summary: A method for combining CRISPR/Cas9 genome editing and the Gibson Assembly® Method to seamlessly assemble large DNA constructs.

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DNA mutations and copy number alterations identified in FFPE tumor samples suggest potential therapeutic targets

Citation summary: Next generation sequencing was used to identify DNA mutations and copy number alterations in FFPE phyllodes tumor samples. Results were validated with Sanger sequencing and IDT PrimeTime® qPCR Assays.

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Nano-delivery of DsiRNAs results in improved gene silencing and anticancer activity

Citation summary: Find out how a dendrimer-based, targeted nano-delivery system that uses Dicer Substrate RNAs (DsiRNAs) leads to improved gene silencing and anticancer activity in prostate cancer models in vitro and in vivo.

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Overlapping biocontainment strategies for genetically modified E. coli

Citation summary: gBlocks Gene Fragments are used to create codon optimized components of a biocontainment system for genetically modified E. coli.

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Validating sequencing results of a repetitive element with digital droplet PCR

Citation summary: Mobilization of long interspersed element 1 (L1) can be involved in human disease and cancer. Read how the specificity and sensitivity of digital droplet PCR can be used to validate the detection of rare L1 insertion events.

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A protocol for using gBlocks Gene Fragments to implement CRISPR/Cas9 genome editing in cell culture

Citation summary: A complete protocol from the Dr George Church lab that describes the design of gRNAs using gBlocks Gene Fragments.

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Advantageous properties of Dicer-Substrate Interfering RNA (DsiRNA) enable improved RNAi-based gene silencing

Citation summary: Comparison of cononical siRNA design to DsiRNAs for performance in siRNA processing and RISC assembly, leading to stronger silencing efficacy.

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Double-quenched probes increase sensitivity of qPCR assay detecting viral load

Citation summary: Use of a ZEN™ Double-Quenched Probe results in a marked decrease in background fluorescence compared to an identical TaqMan® probe containing only a single quencher. The data suggest that such double-quenched probes may be a better approach for other qPCR probe-based assays.

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Decoding Cas9 orthologs using gBlocks® Gene Fragments

Citation summary: gBlocks Gene Fragments were used to create Cas9 orthologs as well as tracRNA expression cassettes for each ortholog tested.

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A high throughput, high resolution melting protocol for identifying single-nucleotide polymorphisms

Citation summary: Rapid SNP evaluation by HTP melt-curve analysis, using gBlocks® Gene Fragments as melt-curve controls.

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Nanoparticle delivery of TNF-α targeting DsiRNA as treatment for rheumatoid arthritis

Citation summary: DsiRNA-loaded PLGA nanoparticles mediate dose-dependent silencing of TNF-α in vitro.

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A fast, sensitive, cost-effective alternative to radiolabeling and HPLC/MS for measuring dNTPs

Citation summary: A rapid and sensitive fluorescence-based method that uses synthetic templates (IDT oligonucleotides), a PrimeTime® Primer, and ZEN™ Double-Quenched Probes for quantifying cellular dNTPs.

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Caffeine addicted bacteria

Citation summary: Use of IDT gBlocks® Gene Fragments in Gibson Assembly® reactions to generate some of the plasmid constructs in this work.

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Barcoding living cell populations with zinc-finger DNA-binding domains

Citation summary: IDT gBlocks® Gene Fragments were used to generate surface zinc-finger DNA-binding domains for barcoding distinct cell lines. These live cells could then be tracked within a heterogeneous mix of cells.

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Predictable control of gene expression by mRNA, 3’ untranslated region motifs

Citation summary: See how these researchers use gBlocks® Gene Fragments as qPCR standards to generate DNA standard curves for absolute quantification of mRNA.

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In vivo delivery of aptamer–DsiRNA molecules targeting HIV-1

Citation summary: Learn how these scientists improve on in vivo siRNA delivery using a modified aptamer-DsiRNA (Dicer-substrate RNA; IDT) molecule to inhibit HIV-1 replication.

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Small RNA regulation of guanine quartet formation and antigenic variation

Citation summary: Using gBlocks® Gene Fragments, these researchers create mutated version of a small RNA to show how it facilitates the formation of a guanine quartet.

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Mimicking heredity and evolution using xeno-nucleic acid aptamers

Citation summary: The researchers engineered polymerases that can synthesize XNA from a DNA template and reverse transcribe XNA back into DNA. Results showed high affinity and specificity of target binding, demonstrating the capacity of XNAs for Darwinian evolution.

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qPCR validation of microarray data using PrimeTime® qPCR Assays and ZEN™ Double-Quenched Probes

Citation summary: See this example of the use of ZEN™ Double-Quenched Probes with PrimeTime® qPCR Assays for qRT-PCR experiments validating microarray data,

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Studying protein interactions with gBlocks® Gene Fragments

Citation summary: Read how scientists use a fragment of adenomatous polyposis coli protein (APC), expressed from a gBlocks Gene Fragment, to monitor interactions of an axin mutant and wild-type RGS domain with this protein. The results provide a better understand of axin’s role in the β-catenin destruction complex, part of a Wnt signaling pathway.

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Mutagenesis using gBlocks® Gene Fragments

Citation summary: Learn how just 3 synthetic, high fidelity, double-stranded gBlocks Gene Fragments used to mutate 18 different sites over the entire exon 7, 1039 bp sequence.

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Quick oligonucleotide assembly

Citation summary: A protocol for assembling high-fidelity Ultramer™ oligonucleotides with overlapping sequences into large constructs using Saccharomyces cerevisiae.

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