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Scientific Publications Referencing IDT Products

Efficient Multiplex Processing of Pooled Barcoded Samples

Harakalova M, Mokry M, et al. (2011) Multiplexed array-based and in-solution genomic enrichment for flexible and cost-effective targeted next-generation sequencing. Nature Protocols, 6(12):1870–1886.

Advances in next generation sequencing (NGS) technologies enable high throughput sequencing of complete genomes and protein-coding regions (exomes). Because the costs of NGS remain relatively high for most research labs and the interpretation of the resulting data is a challenge, requiring vast amounts of computational resources, many researchers seek methods to sequence only specific regions of interest within the genome. To achieve this, researchers are making increased use of targeted genomic enrichment techniques. Harakalova et al. describe a cost-effective procedure for parallel genomic library preparation from multiple samples followed by targeted enrichment of pooled barcoded samples. The protocol is compatible with microarray- and solution-based capture methods. Using barcoded adapters and blocking oligonucleotides manufactured by IDT, the researchers were able to multiplex 3–5 samples for whole exome analysis, 20 samples for 5 Mb targets, and 96 samples for targets of 0.4 Mb.