Oligonucleotides are hydrophilic and often have difficulty permeating cell membranes. Cholesterol is hydrophobic, and when conjugated to oligonucleotides, can facilitate uptake into cells. It has been used as a transfection aid for antisense oligos and siRNAs, both in vitro and in vivo. The TEG (15 atom triethylene glycol) spacer further increases cellular uptake.
Availability: DNA and RNA
Scales: 100 nmol to 10 𝝁mol
Purification: HPLC required
IDT Ordering Symbol: /3CholTEG/
Figure 1. Cholesterol-TEG. Cholesterol-TEG can be used to modify both DNA and RNA oligos on scales from 100 nmol to 10 μmol. Because cholesterol is difficult to manufacture, it requires HPLC purification. To order, use the symbol /3CholTEG/.
Author: Jeremy Pritchard is a Technical Support Representative at IDT.
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