Photo-cleavable (PC) modifications contain a photolabile functional group that is cleavable by UV light of specific wavelength (300–350 nm). The PC spacer is a 10-atom linker arm that can only be cleaved when exposed to UV light within the appropriate spectral range. The resulting oligo will have a 5’ phosphate group that is available for subsequent ligase reactions. PC spacers can be placed between DNA bases or between an oligo and a terminal modification such as a fluorophore. IDT also offers 5’ PC Biotin (/5PCBio/), which allows for post-binding release and the design of controlled capture-and-release assays.
To order, select PC Spacer or PC Biotin from the 5’ Mods or Internal Mods tab on the oligo order page. Note that this modification requires HPLC purification.
Author: Jeremy Pritchard is a Technical Support Representative at IDT.
Disclaimer: PC linkers used in commercial applications require licensing from Ambergen, Inc. (www.ambergen.com).