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Product Spotlight
Your Favorite Products and Their Applications

A recombinant Cas9 enzyme that drastically reduces CRISPR off-target effects

Learn about the Cas9 variant, Alt-R® S.p. HiFi Cas9 Nuclease 3NLS, that greatly reduces off-target cutting events during CRISPR genome editing. At the same time, it maintains the high level of on-target editing efficiency as wild-type Cas9 nuclease.

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Synthetic CpG ODNs activate immune cells through the Toll-like receptor (TLR) pathway

Did you know that synthetic CpG oligodeoxynucleotides (ODNs) can serve as an adjuvant to enhance the immune response of vaccines by mimicking the immune-stimulatory effects of unmethylated bacterial or viral sequences? Read about the 3 classes of CpG ODNs and how their distinct structures are tied to their varied functions. Order these modified oligos from IDT.

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Use of template switching oligos (TS oligos, TSOs) for efficient cDNA library construction

Conventional cDNA construction strategies usually result in an underrepresentation of the 5' ends of cDNA. However, use of a template switching chimeric DNA:RNA oligo and MMLV reverse transcriptase can improve on this. See how this approach, dubbed SMART, makes it possible to efficiently amplify the entire full-length transcript pool, in a completely sequence-independent manner.

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Long, custom RNA oligos—Ultramer® RNA Oligonucleotides

Product spotlight: Use Ultramer® RNA Oligonucleotides to increase specificity and improve performance in a variety of RNA-related applications. Learn more about these high-quality, single-stranded custom oligos, made up to 120 bases.

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qPCR with intercalating dyes: PrimeTime® qPCR Primers

Need qPCR assays to use with intercalating dyes? You can obtain PrimeTime qPCR Primers to detect genes in human, mouse, and rat transcriptomes with intercalating dyes such as SYBR® Green and EvaGreen®. Because our primer only and probe-based assays use the same designs, when you decide to include probes in your assays, you can just request the probes that go along with these same primer sequences.

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Generate codon balanced libraries for mutagenesis with trimer modifications

Incorporating oligo codon trimers into oligo libraries results in balanced encoding of amino acids and eliminates unwanted stop codons. Such oligo libraries are useful for mutagenesis experiments to prepare proteins for screening for potential improvements in biological function.

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N6-methyladenosine (m6A)—modulating RNA localization, structure, stability, splicing, and translation

Modification Highlight: Read about the mechanism of methylation and functions of N6-methyladenosine (m6A) modifications in the cell. N6-methyladenosine is available as a popular non-catalog modification from IDT, providing a substrate for studying the role of this natural, reversible, post-translational modification.

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Valuable support for genomics research

Product Spotlight: Take advantage of the full list of products, services, and support IDT provides for genomics research. Whether you are performing sequencing, gene expression, genome editing, or synthetic biology experiments, IDT has cost-effective, rapid, high quality, and high throughput solutions.

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Insert an abasic site into your sequence

Use the dSpacer, rSpacer, and Abasic II modifications to introduce abasic sites into DNA or RNA oligonucleotides. These modifications create a single base space that replicates the loss of base pairing ability by a nucleotide.

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Need a library of related DNA or RNA oligo sequences?

Build variability into your oligo sequences by incorporating Mixed Bases. We offer mixes of multiple base types as well as nonstandard and modified bases.

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Increase the Tm of short, AT-rich primers and probes

Modification highlight: Add this modified base to increase the melting temperature (Tm) of primers and probes. It is especially useful when you need to work with short A-T rich sequences.

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Inverted bases

Learn how inverted bases allow you to reverse the orientation of part of your oligo sequence or add a 5-end restricted modification to the 3 end of your sequence.

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Improve target capture using xGen® Lockdown® Probes and Reagents with an optimized protocol

xGen Lockdown Reagents and new hybridization capture protocol for xGen Lockdown Probes and Panels reveal the increased potential of IDT target enrichment products.

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Getting started with Alt-R® CRISPR-Cas9 genome editing

Webinar summary: Learn about the components of the Alt-R® CRISPR-Cas9 System for improved genome editing. Get information on designing Alt-R CRISPR crRNA oligos, and review the genome editing protocol from the user guide.

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Consistent, high performance genome editing

Product spotlight: Looking to improve the performance of your CRISPR-Cas9 genome editing application? The Alt-R™ CRISPR-Cas9 System offers potent on-target editing, easy implementation, and reduced cellular toxicity.

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Obtain high efficiency qPCR results using PrimeTime® Gene Expression Master Mix

Product Spotlight: If you are doing 5′ nuclease assays for qPCR or 2-step qPCR, use this versatile master mix in both singleplex or multiplex. This master mix is compatible with a wide range of instruments—whether you prefer standard or fast cycling conditions, and whether or not you need a reference dye.

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Design functional oligos with poly G runs

Modification Highlight: Insert this modification in a run of G residues to increase oligo yield and purity, eliminate secondary structure, improve probe-based qPCR signal, and increase duplex stability and mismatch discrimination.

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Increase sensitivity and precision in your qPCR experiments

Learn how you can use double-quenched probes to decrease background, and increase sensitivity and precision in your qPCR experiments.

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Plates of custom DNA fragments for high-throughput applications

Obtain gBlocks Gene Fragments in 96-well plates to facilitate large orders and high throughput use.

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Get the most out of your NGS samples—expandable tumor and disease target capture panels

Learn about expandable NGS target capture panels that enrich for mutated genes implicated in tumors and genes associated with inherited diseases. Designed in collaboration with experts from the Emory Genetics Laboratory and the Cancer Genome Atlas, both panels return consistent results with high reproducibility and deep uniform coverage.

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Generate consistent, reliable exome sequencing results

Spanning 39 Mb of the human genome, the xGen® Exome Research Panel was designed to provide uniform and specific coverage of the coding regions for 19,396 genes. You can easily and cost-effectively expand this panel to include specific non-coding target regions through addition of xGen® Lockdown® Probes.

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MGB Eclipse® Probes for human in vitro diagnostics

Product spotlight: Obtain MGB Eclipse® Probes for human in vitro diagnostic end-use applications. Selecting MGB Eclipse® Probes made by the IDT GMP manufacturing division provides you with complete process transparency and product traceability, in addition to consistent, reliable oligonucleotide quality.

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gBlocks® Gene Fragments Ordering Tool

Easy tool for uploading or entering gBlocks Gene Fragments sequence requests, that also judges complexity, and allows you to edit the sequences on the spot.

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Longer gBlocks® Gene Fragments make gene assembly simple

Learn how you can use made-to-order, double-stranded DNA fragments up to 3 kb to simplify your cloning and mutagenesis protocols.

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Get just the targets you need in NGS capture panels

There are several disadvantages to purchasing fixed target capture panels or having custom panels manufactured. A solution is to create your own custom NGS target capture panels from stocked human gene probe pools.

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RxnReady Oligos—premixed oligos for PCR and more

Would getting your oligos premixed help speed up your research? Have 2 standard desalted DNA oligonucleotides premixed in a single tube according to your specifications. This can be useful when performing PCR, fluorescent dye RT-PCR, or when generating sets of insertions or deletions through site-directed mutagenesis.

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Fluorescent dyes with no licensing restrictions—a growing portfolio

Need fluorescent dyes suitable for commercial and diagnostic applications? These have no patent licensing restrictions. Review this table of Freedom Dye alternatives for commonly used proprietary dyes.

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Targeted sequencing for acute myeloid leukemia

Use this NGS target enrichment panel, composed of >11,500 xGen Lockdown® Probes, to sequence more than 260 genes associated with the acute myeloid leukemia (AML) disease pathway.

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Accelerate your recombinant gene or protein development

Library of variant dsDNA sequences to generate up to 4E18 sequence variations that could be used for a variety of functional screenings of nucleotide or peptide variants.

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Libraries of double-stranded DNA fragments

Learn about obtaining double-stranded DNA fragment libraries that contain up to 18 consecutive N or K bases for generating up to 4e18 sequence variations.

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qPCR probes—get the right scale at the right price

Only running 100–500 amplification reactions with FAM-labeled probes? Get a better price by using the PrimeTime Eco probes mid-range reaction scale for researchers. It's cheaper per reaction than larger reaction sizes and provides double-quenched FAM probes.

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qPCR assay plates for large and high throughput studies

Customize qPCR assay plates for probe-based or primer alone assays. You can mix different dye-quencher combinations and request replicate plates. And no need to fill every well.

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Alternative dyes for FISH, FRET, and qPCR

Use ATTO™ dye labeled oligonucleotides as alternatives in applications including fluorescence in situ hybridization (FISH), fluorescence resonance energy transfer (FRET), and dual-labeled probes used in qPCR experiments.

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Making RNAi Research Easy

IDT can supply any of the forms of interfering RNA you need.

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Need Small Amounts of Many Oligos?

DNA Plate Oligos are now available in 500 pmole amounts.

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Inhibiting miRNAs using antisense oligonucleotides

Learn the basics about miRNA Inhibitors (also known as anti-miRNA oligos, or AMOs) for knockdown of miRNA expression in loss/gain of function studies.

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5-Hydroxymethyl-dC for epigenetic research

Naturally occurring 5-hydroxymethylcytosine (5-hmC) has been hypothesized to be an intermediate in a demethylation pathway or as an additional epigenetic factor. Learn more about this exciting new modification.

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Assembling gene fragments using isothermal assembly

Learn how to use the Gibson Isothermal Assembly method to quickly combine gene fragments, such as IDT gBlocks Gene Fragments, into large constructs.

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Easy gene assembly—gBlocks® custom dsDNA gene fragments

Could your research benefit from faster gene assembly? gBlocks Gene Fragments are 125—3000 bp, custom double-stranded DNA fragments of your specified sequence. Use them for all your cloning applications.

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200 pmol Ultramer® DNA Plate Oligo

Ultramer high-fidelity, long oligonucleotides now available in 200 pmol plates.

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More ZEN/Fluorophore Combos!

Including a second quencher in one's qPCR probe will increase assay sensitivity and provide improved precision.

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qPCR probes—achieve high Tm without sacrificing quenching

Need a longer probe, or one with a higher Tm? Placed directly between DNA bases, the ZEN Internal Quencher eliminates the need for LNAs, MGB, or internal quenchers attached to thymidine bases. When combined with a 3’ quencher, you can design longer probes with sufficient melting temperature for qPCR while maintaining a consistently low background.

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Click chemistry-generated, internal dye-labeled oligonucleotides

Learn about "click chemistry" reactions, used to join small chemical subunits in a modular fashion, yielding singular reaction products that are typically physiologically stable and stereospecific.

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Using qPCR assays in multiplex experiments

Product spotlight: Starting multiplex qPCR experiments? IDT PrimeTime Predesigned and Custom qPCR Assays offer multiple dye/quencher combinations and primer/probe ratios to simplify the multiplex experiment design.

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Ultramer Oligonucleotides

Ultramers and miniGenes as positive controls

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SYBR® to 5' nuclease assays

Product spotlight: Researchers often use intercalating dyes, like SYBR® Green I, for qPCR experiments for the advantage they bring in lowered price and reduced turnaround time. However, the disadvantages to using these reagents are significant. Read how easy it is to convert to 5’ nuclease assays and the advantages the addition of a hydrolysis probe will provide.

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LNA Calculator Updates in OligoAnalyzer

The free, online OligoAnalyzer tool is used to analyze the physical properties of oligonucleotide sequences.

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PrimeTime® Predesigned qPCR Assays

PrimeTime Predesigned qPCR Assays are now available for human, mouse, and rat transcriptomes in the NCBI database.

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