New nearest-neighbor parameters correctly forecast the positive and negative effects of LNAs on mismatch discrimination. Specificity is enhanced in a majority of sequences and is dependent on mismatch type and adjacent base pairs.
Ultramer Oligonucleotides were used as standards due to their purity and quantification precision.
An IDT MiniGene expressing wild-type survival motor neuron from SMN2, and a second SMN2 MiniGene with a silent mutation in the siRNA recognition sequence, were used to study the effects of siRNA knockdown, and rescue, of SMN1/2 on gene splicing. IDT DsiRNA Duplexes were also used for the siRNA knockdown experiments.
A review summarizing the use of cell-internalizing aptamers to deliver siRNAs to target cells. Highlights the optimization and improvement of aptamer-targeted siRNAs for clinical translation.
Dicer-substrate RNA (DsiRNA) is used to target physiological pain modulators in animal models for in vivo pain research.
Gantier MP, Irving AT, Kaparakis-Liaskos M, Xu D, Evans VA, Cameron PU, Bourne JA, Ferrero RL, John M, Behlke MA, Williams BR.
Genetic modulation of TLR8 response following bacterial phagocytosis
In situ detection of mature microRNAs via labeled extension of Ultramer templates is used to detect human miR-212 expression patterns.
Use of a novel CpG-motif oligonucleotide delivery tool to deliver anti-STAT3 DsiRNA to TLR9-positive cells, where Dicer cleavage releases the mature siRNA from the CpG ligand.