Immunoprecipitated DNA was analyzed by real-time PCR using IDT PrimeTime qPCR Assays containing a ZEN Double-Quenched Probes and primers.
gBlocks Gene Fragments were used to generate mutant autonomously replicating sequences, or ARSs, associated with replication origins in yeast chromosomal DNA.
A gBlocks Gene Fragment was used to create an sgRNA expression vector for use with the CRISPR/Cas9 system.
gBlocks Gene Fragments were used to create sgRNA expression plasmids.
An RT-qPCR assay for HCV quantification was performed with probe-based assays using IDT primers and ZEN double-quenched probes.The assay amplified the 5′ UTR of the HCV genome and provided reduced background and increased signal.
The authors explain how next generation sequencing has advanced mitochondrial genetics research. They note how IDT’s xGen Lockdown Probes can be used to accomplish solution phase capture of mtDNA.
5 gBlocks Gene Fragments were used to assemble the ~2 kb coding sequence for Micalcl (NM_027587).
Sarvestani ST, Tate MD, Moffat, JM, Jacobi AM, Behlke, MA, Miller AR, Beckham SA, McCoy CE, Chen W, Mintern JD, O’Keeffe M, John M, Williams BR, Gantier MP.
Inosine-mediated modulation of RNA sensing by Toll-like receptor 7 (TLR7) and TLR8
α6A and α6B were amplified using IDT PrimeTime qPCR Assays. The assays were composed of primers and double-quenched hydrolysis probes containing the 5′ fluorophore FAM, and ZEN and IABkFQ quenchers. Relative mRNA levels were established by normalization to a pool of cDNA and calculated according to the Pfaffl mathematical model.
Dudek H, Wong DH, Arvan R, Shah A, Wortham K, Ying B, Diwanji R, Zhou W, Holmes B, Yang H, Cyr WA, Zhou Y, Shah A, Farkiwala R, Lee M, Li Y, Rettig GR, Collingwood MA, Basu SK, Behlke MA, Brown BD.
Knockdown of β-catenin with Dicer-substrate siRNAs reduces liver tumor burden in vivo
The scientists combined reverse transcription with RNase H2-dependent qPCR using blocked cleavable primers to measure levels of alternatively spliced transcripts of latrophilins and of teneurins.
FAM-labeled and Cy5.5-labeled probes were synthesized using standard solid-phase phosphoramidite chemistry and followed by HPLC purification. The Cy5.5-labeled probes included ZEN internal quencher, Iowa Black quenchers or inverted dT on the 3′ ends, and amine on the 5′ ends. Probe identities were confirmed using electron spray ionization mass spectrometry (ESI-MS).
Hernandez FJ, Huang L, Olson ME, Power KM, Hernandez LI, Meyerholz DK, Thedens DR, Behlke MA, Horswill AR, JcNamara JO 2nd.
Noninvasive imaging of Staphylococcus aureus infections with a nuclease-activated probe
gBlocks Gene Fragments were used to generate codon-optimized cytochrome P450 enzymes, and other hemoproteins, in order to study their catalytic use in non-natural olefin cyclopropanation reactions.
gBlocks Gene Fragments were used to construct a codon optimized Cas9 sequence for expression in Drosophila. Optimization of the sequence was performed using the IDT Codon Optimization tool.
gBlocks Gene Fragments were used to create sgRNA, gene specific sequences.