The scientists combined reverse transcription with RNase H2-dependent qPCR using blocked cleavable primers to measure levels of alternatively spliced transcripts of latrophilins and of teneurins.
FAM-labeled and Cy5.5-labeled probes were synthesized using standard solid-phase phosphoramidite chemistry and followed by HPLC purification. The Cy5.5-labeled probes included ZEN internal quencher, Iowa Black quenchers or inverted dT on the 3′ ends, and amine on the 5′ ends. Probe identities were confirmed using electron spray ionization mass spectrometry (ESI-MS).
Hernandez FJ, Huang L, Olson ME, Power KM, Hernandez LI, Meyerholz DK, Thedens DR, Behlke MA, Horswill AR, JcNamara JO 2nd.
Noninvasive imaging of Staphylococcus aureus infections with a nuclease-activated probe
gBlocks Gene Fragments were used to generate codon-optimized cytochrome P450 enzymes, and other hemoproteins, in order to study their catalytic use in non-natural olefin cyclopropanation reactions.
gBlocks Gene Fragments were used to construct a codon optimized Cas9 sequence for expression in Drosophila. Optimization of the sequence was performed using the IDT Codon Optimization tool.
gBlocks Gene Fragments were used to create sgRNA, gene specific sequences.
Researchers at the Foundation for Applied Molecular Evolution (Gainesville, FL, USA) use Ultramer DNA Oligos in experiments demonstrating the practical improvements that can be made to recombinase polymerase amplification (RPA), through addition of self-avoiding molecular recognition systems (SAMRS) to RPA-primers.
Bestas B, Moreno PM, Blomberg KE, Mohammad DK, Sutlu T, Nordin JA, Guterstam P, Gustafsson MO, Kharazi S, Piatosa B, Roberts TC, Behlke MA, Wood MJ, Gait MJ, Lundin, KE, El Andaloussi S, Mansson R, Berglof A, Wengel J, Smith CI.
Splice-correcting oligonucleotides restore BTK function in X-linked agammaglobulinemia model
J Clin Invest,
gBlocks Gene Fragments were used to generate codon-optimized acRAF genes from <em>H. neapolitanus</em> and <em>P. marinus</em>.
gBlocks Gene Fragments were used as positive genotype controls for the described 5′ nuclease, SNP genotyping assay.
A PrimeTime Mini qPCR Assay was used for detection of 18S rRNA (PrimeTime Std qPCR Assay) and NBR1 (Mm.PT.45.6651111) in tissue from the striatum and cortex of wild type and R6/1 mice.
gBlocks Gene Fragments were used for creating sgRNA expression constructs for targeting mutant Cas9 variants to test either transcription activation or genome modification.
gBlocks Gene Fragments were used to create an Mxi1 transcription repressor domain and assembled with a dCas9 construct using the Gibson Assembly™ method into an expression vector. The resulting chimeric, transcriptional repressor was then shown to be targetable using the CRISPR/Cas9 mechanism.