Efficient, noncovalent binding of Dicer-substrate siRNAs (DsiRNAs) to an aptamer for effective in vivo knockdown of target mRNAs and potent inhibition of HIV-1 replication.
The scientists used IDT PrimeTime qPCR Assays for mouse genotyping.
PrimeTime qPCR Primers and the intercalation dye, BRYT Green dye, were used to determine the initial amount of RNA present in samples. This amount was normalized using a B-actin endogenous control assay. The PrimeTime Primers utilized were designed to span the intron splice junction (thus avoiding amplification of genomic DNA) between IL-1β exon locations 2–4, BDNF exon locations 2–5, NR1 exon locations 2–3, NR2C exon locations 6–7, and β-actin exon locations 5–6. Gene expression levels were calculated from the qPCR data by normalizing the amplification rate of the target gene's expression against the amplification rate of β-actin, using the DART-PCR method, which does not assume a perfect amplification efficiency for all cycles.
Ramachandran S, Karp PH, Osterhaus SR, Jiang P, Wohlford-Lenane C, Lennox KA, Jacobi AM, Praekh K, Rose SD, Behlke MA, Xing Y, Welsh MJ, McCray PB Jr.
Post-transcriptional regulation of cystic fibrosis transmembrane conductance regulator expression and function by microRNAs
Am J Respir Cell Mol Biol,
IDT PrimeTime qPCR Assays were used to determine the proportion of Staphylococcus aureus to total bacteria populations in individuals with chronic rhinosinusitis.
PNA (peptic nucleic acid-locked nucleic acid) clamp Primers and LNA (locked nucleic acid) mutant probes from IDT were used in a PNA-LNA PCR clamp assay for EGFR mutation analysis.
Tetreault P, Beaudet N, Perron A, Belleville K, Rene A, Cavelier F, Martinez J, Stroh T, Jacobi AM, Rose SD, Behlke MA, and Sarret P.
Spinal NTS2 receptor activation reverses signs of neuropathic pain
gBlocks Gene Fragments are used to generate constructs for yeast two-hybrid assays.
Goraczniak R, Wall BA, Behlke MA, Lennox KA, Ho ES, Zaphiros NH, Jakubowski C, Patel NR, Zhao S, Magaway C, Subbie SA, Jenny Yu L, Lacava S, Reuhl KR, Chen S, Gunderson SI..
U1 adaptor oligonucleotides targeting BCL2 and GRM1 suppress growth of human melanoma xenografts in vivo
Mol Ther Nucleic Acids,
The authors have developed a multiplex PCR method that allows for quantitative analysis of T- and B- cell receptor diversity using next generation sequencing. gBlocks Gene Fragments are used as templates for optimization of the multiplex primer mix, a critical step for maintaining quantifiable differences and detecting low level transcripts.
Ramachandran S, Karp PH, Jiang P, Ostedgaard LS, Walz AE, Fisher JT, Keshavjee S, Lennox KA, Jacobi AM, Rose SD, Behlke MA, Welsh MJ, Xing Y, McCray PB Jr.
A microRNA network regulates expression and biosynthesis of wild-type and DeltaF508 mutant cystic fibrosis transmembrane conductance regulator
Proc Natl Acad Sci USA,
Janas MM, Wang B, Harris AS, Aguiar M, Shaffer JM, Subrahmanyam YV, Behlke MA, Wucherpfennig KW, Gygi SP, Gagnon E, Novina CD.
Alternative RISC assembly: binding and repression of microRNA-mRNA duplexes by human Ago proteins
Use of RNase H2–dependent PCR to amplify and detect the tcdB gene in a region of the pathogenicity locus of C. difficile, with unknown sequence variation and insertions/deletions.