Ultramer Oligonucleotides were used to construct calibration standards. Each Ultramer contained a target sequence from one of the transcripts under study. All qPCR assays included a set of 3 Ultramer calibrators.
Use of DsiRNAs to inhibit TNF-ɑ secretion in the study of inflammatory diseases. Data shows greater effectiveness of 27mer DsiRNAs than 21mers.
Bladder cancer cells pre-treated with Dicer-substrate siRNA (DsiRNA) against Ki-67, a DNA-binding protein associated with cell proliferation proliferation, promotes cell cycle arrest and enhances curcumin-induced apoptosis.
Initiation of isothermal helicase-dependent amplification of a rpoB gene sequence by RNase H2-mediated cleavage of blocked DNA primers.
IDT MiniGene constructs were used as microRNA (miR) sponges against miR-17, miR-18a, miR-19, and miR-92. The sequences were also designed with 5′ Xho1 and 3′ EcoR1 sites for subcloning into other vectors by restriction digest.
Thiel WH, Bair T, Peek AS, Liu X, Dassie J, Stockdale KR, Behlke MA, Miller FJ, Giangrande PH. .
Rapid identification of cell-specific, internalizing RNA aptamers with bioinformatics analyses of a cell-based aptamer selection
Dallas A, Ge Q, Ilves H, Kumar P, Shorenstein J, Kazakov SA, Cuellar TL, McManus MT, Behlke MA, Johnston BH.
Right- and left-loop short shRNAs have distinct and unusual mechanisms of gene silencing
Nucleic Acids Res,
Huang YZ, Hernandez FJ, Gu B, Stockdale KR, Nanapaneni K, Scheetz TE, Behlke MA, Peek AS, Bair T, Giangrande PH, McNamara JO..
RNA aptamer-based functional ligands of the neurotrophin receptor, TrkB
Weinstein S, Emmanuel R, Jacobi AM, Abraham A, Behlke MA, Sprague AG, Novobrantseva T, Nagler, A, Peer D.
RNA inhibition highlights Cyclin D1 as a potential therapeutic target for Mantle Cell Lymphoma
Use of DsiRNAs to inhibit viral replication of metapneumovirus in vitro and in vivo. The authors report highly specific inhibition achieved without induction of cytokines or off-target effects.