In an example of the evolution of DNA-based nanomachinery, researchers devise 2 anti-parallel DNA origami filaments that undergo step-wise, reversible sliding in opposite directions. Movement is powered by DNA fuels mediated by gold nanocrystals.
The researchers address the incorporation and accessiblitiy of DNA strands in DNA origami nanostructures with molecular resolution using DNA-PAINT super resolution microscopy. This method can provide feedback to refine design and assembly of these structures.
The authors use DNA origami with GFP antibodies to provide a system for calibrating fluorophore and antibody labeling efficiency. The method should allow researchers to quantify protein copy number within cells using super-resolution microscopy.
The authors create DNA origami nanostructures that undergo light-induced conformational changes. Two linked 14-helix origami bundles form a chiral object with a tunable angle. A photo-responsive, azobenzene-modified DNA segment is added to the template and upon illumination, is converted to a cis-form, altering the angle of the bound origami bundles.
The authors identify a previously unknown stimulatory mechanism (through RAD51 paralog proteins) for homologous recombination--filament remodeling. They use DNA origami nanostructures to elucidate the mechanism by which RAD51 paralogs enhance homologous recombination.