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5 Citations found

Jensen S, Charles JR, et al. (2020) A sorghum practical haplotype graph facilitates genome-wide imputation and cost-effective genomic prediction. Plant Genome. doi: 10.1002/tpg2.20009

Researchers developed a database called the Practical Haplotype Graph (PHG) to store haplotype and variant information to manage large genomic datasets for breeding programs. rhAmpSeq amplicon sequencing was used to identify single nucleotide variants (SNPs) which were compared to those predicted by the PHG.

Zou C, Karn A, et al. (2020) Haplotyping the Vitis collinear core genome with rhAmpSeq improves marker transferability in a diverse genus . Nature Communications. doi: 10.1038/s41467-019-14280-1

Agriculture uses molecular markers to accelerate transfer of traits when breeding. The researchers developed a low-cost DNA marker system for the grape genus (Vitis) using the rhAmpSeq amplicon sequencing system.

Shapiro J, Iancu O, et al. (2020) Increasing CRISPR efficiency and measuring its specificity in HSPCs using a clinically relevant system. Mol Ther Methods Clin Dev. doi: 10.1016/j.omtm.2020.04.027

This study optimized CRISPR-Cas9 genome editing in an ex vivo system using CD34+ hematopoietic stem and progenitor cells. The researchers created a clear workflow to precisely identify off-target effects using the rhAmpSeq amplicon sequencing system.

Basar R, Daher M, et al. (2020) Large-scale GMP-compliant CRISPR-Cas9–mediated deletion of the glucocorticoid receptor in multivirus-specific T cells. Blood Adv. doi: 10.1182/bloodadvances.2020001977

In this research study, CRISPR-Cas9 was used to delete the glucocorticoid receptor gene in viral specific T cells (VSTs). The gene editing protected the VSTs from lymphocytotoxicity while retaining the glucocorticoid effectiveness against graft-versus-host disease. Off-target edits were characterized by the rhAmpSeq amplicon sequencing system.

CRISPR-Cas9 was used to correct the sickle mutation in hematopoietic stem and progenitor cells before transplantation into a mouse model of sickle cell disease. GUIDE-seq was used to identify off-target edits and the rhAmpSeq amplicon sequencing system quantified on-target and off-target activity.