3 Citations found

The scientists combined reverse transcription with RNase H2-dependent qPCR using blocked cleavable primers to measure levels of alternatively spliced transcripts of latrophilins and of teneurins.

Use of RNase H2–dependent PCR to amplify and detect the tcdB gene in a region of the pathogenicity locus of C. difficile, with unknown sequence variation and insertions/deletions.

Ao W, Aldous S, et al. (2012) Rapid detection of rpoB gene mutations conferring rifampin resistance in Mycobacterium tuberculosis. J Clin Microbiol, 50 : 2433–2440.

Initiation of isothermal helicase-dependent amplification of a rpoB gene sequence by RNase H2-mediated cleavage of blocked DNA primers.