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A high throughput, high resolution melting protocol for identifying single-nucleotide polymorphisms

Bruzzone CM, Tawadros PS, et al. (2013) Enhanced Primer Selection and Synthetic Amplicon Templates Optimize High-Resolution Melting Analysis of Sin­gle-Nucleotide Polymorphisms in a Large Population. Genet Test Mol Biomarkers, 17(9):675–680.

Citation summary: Review this protocol for rapid SNP evaluation by HTP melt-curve analysis. IDT gBlocks® Gene Fragments provide quick and easily constructed melt-curve controls.

Single nucleotide polymorphisms (SNPs) are commonly studied for their contribution to genetic variation and disease. In this article, Bruzzone et al. present a novel method for evaluating SNPs in a fast, high-throughput protocol. The protocol relies on careful primer design, and a “cloning fragment length double-stranded DNA control template” to verify the identity of homozygous peaks in the melt-curve analysis for SNP detection. Sequence-verified gBlocks® Gene Fragments were an ideal solution for the melt-curve controls in this study, and were central to the SNP analysis.

Published Oct 21, 2013