Single-nucleotide polymorphisms (SNPs) and their associated phenotypes provide crucial information for elucidating gene function, identifying species or individuals, diagnosing diseases caused by gene variation, and selecting targeted therapies. PCR-based genotyping is a fundamental technology in molecular biology and genetics, as it allows labs to quickly and inexpensively determine genotype using high-throughput processing and low sample input.
However, as with all methods, there are limitations to consider. A significant concern for PCR genotyping assays is that the primers and probes can bind to incorrect targets and form primer dimers. At best, these non-specific interactions affect signal levels and create challenges for analyzing your data; however, they can make the data unusable.
In the webinar below, rhAmp SNP Genotyping: A novel approach for improving PCR-based SNP genotyping, Dr Scott Rose presents the groundbreaking technology used in rhAmp SNP genotyping assays that greatly reduces primer dimers and off-target artifacts. The method relies on activation of novel RNA-DNA hybrid primers by an RNase H2 enzyme, only when the primers are hybridized to their specific target. Importantly, the RNase H2 enzyme functions optimally under conditions that are compatible with Taq polymerase activity and cycling, such that there are no additional steps beyond those required for a normal PCR genotyping reaction. Watch the recorded webinar below to find out how the technology works, and how it can improve your genotyping experiments.