Spending all your time cloning? There's an easier (and faster) way...

Custom, double-stranded DNA fragments—gBlocks® Gene Fragments—for cloning, mutagenesis, CRISPR, and more

Cloning genomic or cDNA sequences is time consuming and can be labor intensive. Learn how IDT gBlocks Gene Fragments will save you time and money, letting you move forward with your research and bringing you closer to your next publication.

May 10, 2018

Recombining DNA fragments requires time and resources

Construction of vectors by cloning and PCR can be very time consuming, costly, and labor intensive. While the ever-increasing availability of genomic sequence information simplifies the process of obtaining target DNA fragments, especially through the use of PCR, one still must complete multiple, labor intensive steps to obtain the desired DNA constructs. These include designing primers, performing PCR, cloning the amplified fragment, and screening and sequencing resulting clones. This process of obtaining target fragments and assembly can take several weeks or longer.

Custom DNA fragments can jump-start your research

You can eliminate many of these steps and start experiments more quickly and economically by substituting custom synthesized double-stranded DNA fragments in place of DIY constructs. gBlocks Gene Fragments are sequence verified DNA fragments that can be used in any application requiring double-stranded DNA. They can greatly accelerate your research. Once ordered, the target fragments are available in as few as 2 business days.

Sequence verified

Each gBlocks Gene Fragment undergoes rigorous IDT quality control testing, which includes size verification via capillary electrophoresis and sequence identification by mass spectrometry. In the majority of cases, >80% of recombinant colonies obtained from cloning any gBlocks Gene Fragment will contain the desired insert.

Synthesized up to 3000 bp

The size of DNA fragments used in molecular biology studies vary substantially based on the nature of the research conducted. They can be genomic or cDNA sequences, non-coding RNAs, or aptamers, thus ranging from tens to thousands of base pairs. Expression vectors are one of the most commonly used recombinant DNA molecules. These constructs usually include a short promoter sequence driving the expression of a cDNA fragment followed by a poly(A) tail signal. The first human genome reference sequence reveals that the average size of human mRNA is less than 3000 bp. Hence a single gBlocks Gene Fragment can deliver the entire coding sequence of most human genes.

Compatible with commonly used assembly and cloning kits

gBlocks Gene Fragments are compatible with many convenient assembly and cloning kits, such as the Gibson Assembly® kits (Synthetic Genomics, Inc), NEBuilder® HiFi kits (New England Biolabs), and In-Fusion® cloning kits (Clontech). Thus, they can be seamlessly incorporated into your ongoing research.

Design using the free, online IDT Codon Optimization Tool

While numerous factors contribute to the success of protein expression studies, codon optimization plays a critical role, particularly when these studies are carried out in a heterologous system. This is due to differences in codon usage among different organisms. This preferential use of codons can impact the success of expression studies, thus needs to be taken into consideration. The free, online Codon Optimization Tool will help you rebalance codon usage of your sequence based on the frequencies of each codon’s usage in the chosen organism for protein expression.

Tips for working with gBlocks Gene Fragments

In the following video, Ann Tigges, Synthetic Biology Business Coordinator at IDT, describes what to do when you receive your gBlocks Gene Fragments. Included is practical information on how to resuspend, quantify, and calculate the copy number of your gBlocks Gene Fragments.

To order or obtain more information, visit gBlocks Gene Fragments.

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