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Strategies for optimizing high throughput qPCR for expression profiling

Webinar summary: Learn how to address the challenges of high throughput RT-qPCR expression profiling from a prominent qPCR expert, Dr. Mikael Kubista (TATAA Biocenter, Sweden).

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Real-time, reverse transcription-quantitative PCR (RT-qPCR) is the gold standard for gene expression analysis. This method allows researchers to generate large amounts of data from small sample sizes in just a few hours. These same characteristics should make it an ideal technique for evaluating a large number of samples for hundreds of expression markers. However, careful attention to experimental design and data analysis are needed to ensure that you obtain accurate and precise results from high throughput experiments. These precautions can also save you time and money.

Whether you are new to or experienced with high throughput gene expression profiling, listen to a joint webinar from IDT and the TATAA Biocenter to get valuable tips for designing and analyzing experiments from a prominent qPCR expert. Dr. Mikael Kubista, founder of the TATAA Biocenter and coauthor of the MIQE guidelines [1], shares his knowledge about the major challenges of high throughput expression profiling. Topics include optimizing and validating assays, sample quality testing, merging multi-plate measurements into a common analysis, as well as measuring and compensating for background due to genomic DNA. Along the way, you will learn about advantageous QC products from TATAA Biocenter and robust qPCR assays and controls from IDT.

Research profile

Dr Mikael Kubista is the founder and CEO of the TATAA Biocenter (www.tataa.com) and head of the Department of Gene Expression at the Institute of Biotechnology of the Czech Academy of Sciences. He was a pioneer in the development of quantitative real-time PCR (qPCR) and introduced qPCR for single-cell expression profiling.

Dr Kubista coauthored the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines [1] and is a member of the CEN and ISO workgroups that are drafting the forthcoming technical specifications and guidelines on the preanalytical processes in molecular diagnostics.

At TATAA, he has led the development of high throughput expression profiling and pioneered multiway profiling. Dr Kubista has also developed qPCR tomography for intracellular expression profiling and was in the TATAA team that pioneered multianalyte single-cell profiling.

References

  1. Bustin SA, Benes V, Garson JA (2009) The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem, 55(4):611–622. doi: 10.1373/clinchem.2008.112797.

Author(s)

Maureen Young, PhD, Senior Scientific Writer, IDT.

Published Sep 29, 2015
Revised/updated Aug 24, 2016

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