The CRISPR (clustered regularly interspaced palindromic repeat)/Cas9 genome editing method has many advantages over other genome editing techniques. CRISPR/Cas9 is much easier to implement than engineered nucleases, and the necessary components can be generated using a variety of standard molecular biology methods. However, the various CRISPR strategies perform differently, and choosing the wrong CRISPR reagents can lead to poor editing efficiency, cell toxicity, off-target editing, and increased labor and material costs for the lab.
IDT has developed a simple and affordable CRISPR-Cas9 solution that outperforms other CRISPR reagents. In this recorded webinar, Dr Rolf Turk provides an overview of the advantages of using a Cas9:tracrRNA:crRNA ribonucleoprotein (RNP) complex in genome editing experiments, and explains why it is the most efficient driver for genome editing compared to alternative methods, such as expression plasmids or the use of sgRNAs. Significant attention is given to IDT protocols for RNP delivery, and how researchers can optimize transfection or electroporation methods for delivery of the RNP. Watch the recorded webinar below to find out how the Alt-R® CRISPR-Cas9 System and RNP delivery can simplify and improve your genome editing research.